Assay Detail
Binding
CHEMBL4190136
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of C-terminal His-tagged recombinant human CYP17A1delta19H mutant expressed in Escherichia coli DH5alpha assessed as decrease in progesterone hydroxylation preincubated for 3 mins followed by NADPH addition measured after 10 mins in presence of cytochrome p450 reductase by LC-MS/MS method
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Intermediate |
Target
Steroid 17-alpha-hydroxylase/17,20 lyase (CHEMBL3522) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Structure-Based Design of Inhibitors with Improved Selectivity for Steroidogenic Cytochrome P450 17A1 over Cytochrome P450 21A2.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 11 | 7.81 | 8.31 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL254328 | ABIRATERONE | 3.0 | 1 | 8.31 |
| CHEMBL132778 | — | — | 1 | 8.21 |
| CHEMBL4213452 | — | — | 1 | 8.14 |
| CHEMBL4210737 | — | — | 1 | 8.11 |
| CHEMBL4209916 | — | — | 1 | 7.91 |
| CHEMBL4209941 | — | — | 1 | 7.81 |
| CHEMBL4214976 | — | — | 1 | 7.78 |
| CHEMBL4210141 | — | — | 1 | 7.64 |
| CHEMBL2105738 | GALETERONE | 3.0 | 1 | 7.55 |
| CHEMBL4210952 | — | — | 1 | 6.64 |
| CHEMBL4203137 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL254328 | ABIRATERONE | IC50 | = | 4.94 | nM | 8.31 |
| CHEMBL132778 | — | IC50 | = | 6.13 | nM | 8.21 |
| CHEMBL4213452 | — | IC50 | = | 7.2 | nM | 8.14 |
| CHEMBL4210737 | — | IC50 | = | 7.83 | nM | 8.11 |
| CHEMBL4209916 | — | IC50 | = | 12.4 | nM | 7.91 |
| CHEMBL4209941 | — | IC50 | = | 15.5 | nM | 7.81 |
| CHEMBL4214976 | — | IC50 | = | 16.5 | nM | 7.78 |
| CHEMBL4210141 | — | IC50 | = | 22.8 | nM | 7.64 |
| CHEMBL2105738 | GALETERONE | IC50 | = | 28.1 | nM | 7.55 |
| CHEMBL4210952 | — | IC50 | = | 230.0 | nM | 6.64 |
| CHEMBL4203137 | — | IC50 | > | 20000.0 | nM | - |