Assay Detail
Binding
CHEMBL4193672
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Estrogenic activity at estrogen receptor alpha in human Ishikawa cells assessed as induction of alkaline phosphatase activity using p-Nitrophenol phosphate as substrate pretreated for 96 hrs followed by substrate addition measured every 15 secs by scanning spectrophotometric analysis
12
Total Activities
6
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Ishikawa |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
DESIGNER Extracts as Tools to Balance Estrogenic and Chemopreventive Activities of Botanicals for Women's Health.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Emax | 6 | - | - |
| EC50 | 6 | 7.53 | 9.70 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL135 | ESTRADIOL | 4.0 | 2 | 9.70 |
| CHEMBL376915 | — | — | 2 | 8.15 |
| CHEMBL1537093 | — | — | 2 | 6.40 |
| CHEMBL492828 | ISOXANTHOHUMOL | — | 2 | 5.85 |
| CHEMBL294099 | — | — | 2 | - |
| CHEMBL253896 | XANTHOHUMOL | 2.0 | 2 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL135 | ESTRADIOL | EC50 | = | 0.2 | nM | 9.70 |
| CHEMBL376915 | — | EC50 | = | 7.0 | nM | 8.15 |
| CHEMBL1537093 | — | EC50 | = | 400.0 | nM | 6.40 |
| CHEMBL492828 | ISOXANTHOHUMOL | EC50 | = | 1400.0 | nM | 5.85 |
| CHEMBL294099 | — | Emax | - | — | - | |
| CHEMBL135 | ESTRADIOL | Emax | = | 137.0 | % | - |
| CHEMBL1537093 | — | Emax | = | 57.4 | % | - |
| CHEMBL253896 | XANTHOHUMOL | Emax | - | — | - | |
| CHEMBL492828 | ISOXANTHOHUMOL | Emax | = | 80.7 | % | - |
| CHEMBL376915 | — | Emax | = | 97.1 | % | - |
| CHEMBL294099 | — | EC50 | - | — | - | |
| CHEMBL253896 | XANTHOHUMOL | EC50 | - | — | - |