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Assay Detail

CHEMBL4193976

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of bovine milk xanthine oxidoreductase pre-incubated for 3 mins followed xanthine substrate addition and measured every 1 mins for 10 mins by spectrophotometry
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Bos taurus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Xanthine dehydrogenase/oxidase (CHEMBL3649)
Type SINGLE PROTEIN
Organism Bos taurus

Publication

Synthesis and bioevaluation of 1-phenyl-pyrazole-4-carboxylic acid derivatives as potent xanthine oxidoreductase inhibitors.
Li J, Wu F, Liu X, Zou Y, Chen H, Li Z, Zhang L.
Eur J Med Chem (2017) 140 : 20 -30
PubMed 28918097 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 22 8.04 8.62

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL172682 1 8.62
CHEMBL169526 1 8.46
CHEMBL4203776 1 8.38
CHEMBL1164729 FEBUXOSTAT 4.0 1 8.28
CHEMBL4208142 1 8.24
CHEMBL4217468 1 8.24
CHEMBL4213055 1 8.17
CHEMBL4212512 1 8.11
CHEMBL4205230 1 7.97
CHEMBL4208654 1 7.88
CHEMBL4212094 1 7.64
CHEMBL4217071 1 7.35
CHEMBL4204327 1 7.22
CHEMBL4203394 1 -
CHEMBL4208265 1 -
CHEMBL4207744 1 -
CHEMBL4212653 1 -
CHEMBL4205791 1 -
CHEMBL4217496 1 -
CHEMBL4216546 1 -
CHEMBL4216000 1 -
CHEMBL4207541 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL172682 IC50 = 2.4 nM 8.62
CHEMBL169526 IC50 = 3.5 nM 8.46
CHEMBL4203776 IC50 = 4.2 nM 8.38
CHEMBL1164729 FEBUXOSTAT IC50 = 5.2 nM 8.28
CHEMBL4208142 IC50 = 5.7 nM 8.24
CHEMBL4217468 IC50 = 5.7 nM 8.24
CHEMBL4213055 IC50 = 6.7 nM 8.17
CHEMBL4212512 IC50 = 7.7 nM 8.11
CHEMBL4205230 IC50 = 10.8 nM 7.97
CHEMBL4208654 IC50 = 13.3 nM 7.88
CHEMBL4212094 IC50 = 23.0 nM 7.64
CHEMBL4217071 IC50 = 45.0 nM 7.35
CHEMBL4204327 IC50 = 59.6 nM 7.22
CHEMBL4203394 IC50 > 100.0 nM -
CHEMBL4208265 IC50 > 100.0 nM -
CHEMBL4207744 IC50 > 100.0 nM -
CHEMBL4212653 IC50 > 100.0 nM -
CHEMBL4205791 IC50 > 100.0 nM -
CHEMBL4217496 IC50 > 100.0 nM -
CHEMBL4216546 IC50 > 100.0 nM -
CHEMBL4216000 IC50 > 100.0 nM -
CHEMBL4207541 IC50 > 100.0 nM -