Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL4194884

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to His-tagged BRD4 bromodomain 1 to 2 (K57 to K550 amino acids) (unknown origin) using Alexa647-labeled BET-inhibitor as fluorescent probe by bromodomain TR-FRET binding assay
28
Total Activities
28
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bromodomain-containing protein 4 (CHEMBL1163125)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery and optimization of novel constrained pyrrolopyridone BET family inhibitors.
Fidanze SD, Liu D, Mantei RA, Hasvold LA, Pratt JK, Sheppard GS, Wang L, Holms JH, Dai Y, Aguirre A, Bogdan A, Dietrich JD, Marjanovic J, Park CH, Hutchins CW, Lin X, Bui MH, Huang X, Wilcox D, Li L, Wang R, Kovar P, Magoc TJ, Rajaraman G, Albert DH, Shen Y, Kati WM, McDaniel KF.
Bioorg Med Chem Lett (2018) 28 : 1804 -1810
PubMed 29678460 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 28 7.94 9.21

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4208129 1 9.21
CHEMBL4217457 1 9.06
CHEMBL4212498 1 9.06
CHEMBL3987016 MIVEBRESIB 2.0 1 8.82
CHEMBL4214501 1 8.57
CHEMBL4204316 1 8.57
CHEMBL4213481 1 8.54
CHEMBL4215988 1 8.48
CHEMBL4213045 1 8.39
CHEMBL4206058 1 8.36
CHEMBL4208136 1 8.33
CHEMBL4218411 1 8.32
CHEMBL4202833 1 8.15
CHEMBL4217818 1 8.14
CHEMBL4203764 1 8.11
CHEMBL4212062 1 8.08
CHEMBL4210131 1 7.92
CHEMBL3978817 1 7.89
CHEMBL4215043 1 7.80
CHEMBL4208643 1 7.77
CHEMBL4202497 1 7.58
CHEMBL4217560 1 7.37
CHEMBL3903195 1 7.16
CHEMBL4215025 1 7.10
CHEMBL4207708 1 6.75
CHEMBL4207179 1 6.66
CHEMBL3904546 1 6.17
CHEMBL4203377 1 5.82

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4208129 Ki = 0.62 nM 9.21
CHEMBL4217457 Ki = 0.87 nM 9.06
CHEMBL4212498 Ki = 0.87 nM 9.06
CHEMBL3987016 MIVEBRESIB Ki = 1.5 nM 8.82
CHEMBL4214501 Ki = 2.7 nM 8.57
CHEMBL4204316 Ki = 2.7 nM 8.57
CHEMBL4213481 Ki = 2.9 nM 8.54
CHEMBL4215988 Ki = 3.3 nM 8.48
CHEMBL4213045 Ki = 4.1 nM 8.39
CHEMBL4206058 Ki = 4.4 nM 8.36
CHEMBL4208136 Ki = 4.7 nM 8.33
CHEMBL4218411 Ki = 4.8 nM 8.32
CHEMBL4202833 Ki = 7.1 nM 8.15
CHEMBL4217818 Ki = 7.3 nM 8.14
CHEMBL4203764 Ki = 7.7 nM 8.11
CHEMBL4212062 Ki = 8.4 nM 8.08
CHEMBL4210131 Ki = 12.0 nM 7.92
CHEMBL3978817 Ki = 13.0 nM 7.89
CHEMBL4215043 Ki = 16.0 nM 7.80
CHEMBL4208643 Ki = 17.0 nM 7.77
CHEMBL4202497 Ki = 26.0 nM 7.58
CHEMBL4217560 Ki = 43.0 nM 7.37
CHEMBL3903195 Ki = 69.0 nM 7.16
CHEMBL4215025 Ki = 80.0 nM 7.10
CHEMBL4207708 Ki = 180.0 nM 6.75
CHEMBL4207179 Ki = 220.0 nM 6.66
CHEMBL3904546 Ki = 680.0 nM 6.17
CHEMBL4203377 Ki = 1500.0 nM 5.82