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Assay Detail

CHEMBL4194886

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to BRD4 in human H1299 cells stably expressing E2 and HPV16-LCR luciferase reporter incubated for 24 hrs by Bright-Glo luciferase reporter gene assay based BRD4 engagement assay
28
Total Activities
28
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bromodomain-containing protein 4 (CHEMBL1163125)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery and optimization of novel constrained pyrrolopyridone BET family inhibitors.
Fidanze SD, Liu D, Mantei RA, Hasvold LA, Pratt JK, Sheppard GS, Wang L, Holms JH, Dai Y, Aguirre A, Bogdan A, Dietrich JD, Marjanovic J, Park CH, Hutchins CW, Lin X, Bui MH, Huang X, Wilcox D, Li L, Wang R, Kovar P, Magoc TJ, Rajaraman G, Albert DH, Shen Y, Kati WM, McDaniel KF.
Bioorg Med Chem Lett (2018) 28 : 1804 -1810
PubMed 29678460 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 28 7.66 8.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4212498 1 8.82
CHEMBL4208129 1 8.77
CHEMBL4213481 1 8.64
CHEMBL4215988 1 8.60
CHEMBL4217457 1 8.44
CHEMBL4208136 1 8.43
CHEMBL4213045 1 8.39
CHEMBL4218411 1 8.37
CHEMBL4212062 1 8.34
CHEMBL4204316 1 8.19
CHEMBL4217818 1 8.05
CHEMBL4214501 1 7.75
CHEMBL3987016 MIVEBRESIB 2.0 1 7.70
CHEMBL3978817 1 7.70
CHEMBL4202833 1 7.68
CHEMBL4210131 1 7.64
CHEMBL4215043 1 7.64
CHEMBL4202497 1 7.55
CHEMBL4203764 1 7.51
CHEMBL4206058 1 7.50
CHEMBL4208643 1 7.32
CHEMBL3903195 1 7.24
CHEMBL4217560 1 7.02
CHEMBL4215025 1 6.82
CHEMBL4207708 1 6.72
CHEMBL4207179 1 6.70
CHEMBL3904546 1 5.72
CHEMBL4203377 1 5.30

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4212498 IC50 = 1.5 nM 8.82
CHEMBL4208129 IC50 = 1.7 nM 8.77
CHEMBL4213481 IC50 = 2.3 nM 8.64
CHEMBL4215988 IC50 = 2.5 nM 8.60
CHEMBL4217457 IC50 = 3.6 nM 8.44
CHEMBL4208136 IC50 = 3.7 nM 8.43
CHEMBL4213045 IC50 = 4.1 nM 8.39
CHEMBL4218411 IC50 = 4.3 nM 8.37
CHEMBL4212062 IC50 = 4.6 nM 8.34
CHEMBL4204316 IC50 = 6.5 nM 8.19
CHEMBL4217818 IC50 = 9.0 nM 8.05
CHEMBL4214501 IC50 = 18.0 nM 7.75
CHEMBL3987016 MIVEBRESIB IC50 = 20.0 nM 7.70
CHEMBL3978817 IC50 = 20.0 nM 7.70
CHEMBL4202833 IC50 = 21.0 nM 7.68
CHEMBL4210131 IC50 = 23.0 nM 7.64
CHEMBL4215043 IC50 = 23.0 nM 7.64
CHEMBL4202497 IC50 = 28.0 nM 7.55
CHEMBL4203764 IC50 = 31.0 nM 7.51
CHEMBL4206058 IC50 = 32.0 nM 7.50
CHEMBL4208643 IC50 = 48.0 nM 7.32
CHEMBL3903195 IC50 = 58.0 nM 7.24
CHEMBL4217560 IC50 = 95.0 nM 7.02
CHEMBL4215025 IC50 = 150.0 nM 6.82
CHEMBL4207708 IC50 = 190.0 nM 6.72
CHEMBL4207179 IC50 = 200.0 nM 6.70
CHEMBL3904546 IC50 = 1900.0 nM 5.72
CHEMBL4203377 IC50 = 5000.0 nM 5.30