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Assay Detail

CHEMBL4195142

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human 5-HT1A receptor expressed in CHO-K1 cells co-expressing Galpha16 assessed as decrease in serotonin calcium mobilization preincubated for 25 mins followed by serotonin challenge measured for 30 secs by aequorin-derived luminescence assay
16
Total Activities
8
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO-K1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

5-hydroxytryptamine receptor 1A (CHEMBL214)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and activity of di- or trisubstituted N-(phenoxyalkyl)- or N-{2-[2-(phenoxy)ethoxy]ethyl}piperazine derivatives on the central nervous system.
Pańczyk K, Pytka K, Jakubczyk M, Rapacz A, Sałat K, Furgała A, Siwek A, Głuch-Lutwin M, Gryboś A, Słoczyńska K, Pękala E, Żmudzki P, Bucki A, Kołaczkowski M, Żelaszczyk D, Marona H, Waszkielewicz AM.
Bioorg Med Chem Lett (2018) 28 : 2039 -2049
PubMed 29730027 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 7.07 8.32
Kb 8 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL8618 NAN-190 2 8.32
CHEMBL4209888 2 8.14
CHEMBL38288 2 7.77
CHEMBL4207653 2 7.25
CHEMBL4212812 2 6.54
CHEMBL4213352 2 6.42
CHEMBL4205526 2 6.22
CHEMBL4203310 2 5.93

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL8618 NAN-190 IC50 = 4.73 nM 8.32
CHEMBL4209888 IC50 = 7.19 nM 8.14
CHEMBL38288 IC50 = 16.9 nM 7.77
CHEMBL4207653 IC50 = 56.8 nM 7.25
CHEMBL4212812 IC50 = 291.0 nM 6.54
CHEMBL4213352 IC50 = 376.0 nM 6.42
CHEMBL4205526 IC50 = 595.0 nM 6.22
CHEMBL4203310 IC50 = 1180.0 nM 5.93
CHEMBL4209888 Kb = 1.8 nM -
CHEMBL4205526 Kb = 146.0 nM -
CHEMBL4212812 Kb = 71.5 nM -
CHEMBL4213352 Kb = 92.3 nM -
CHEMBL4207653 Kb = 13.9 nM -
CHEMBL4203310 Kb = 291.0 nM -
CHEMBL8618 NAN-190 Kb = 1.2 nM -
CHEMBL38288 Kb = 6.6 nM -