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Assay Detail

CHEMBL4195359

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiviral activity against APV resistant HIV1 harboring protease L10F/M46I/I50V/I85V mutant infected in human MT4 cells assessed as reduction in p24 Gag protein production after 7 days by chemiluminescent enzyme immunoassay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Human immunodeficiency virus 1
Cell Type MT4
Confidence 1 — Organism
Curated By Intermediate

Target

Human immunodeficiency virus 1 (CHEMBL378)
Type ORGANISM
Organism Human immunodeficiency virus 1

Publication

Design and Synthesis of Highly Potent HIV-1 Protease Inhibitors Containing Tricyclic Fused Ring Systems as Novel P2 Ligands: Structure-Activity Studies, Biological and X-ray Structural Analysis.
Ghosh AK, R Nyalapatla P, Kovela S, Rao KV, Brindisi M, Osswald HL, Amano M, Aoki M, Agniswamy J, Wang YF, Weber IT, Mitsuya H.
J Med Chem (2018) 61 : 4561 -4577
PubMed 29763303 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 6 7.86 9.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4214453 1 9.52
CHEMBL4207145 1 9.49
CHEMBL1163 ATAZANAVIR 4.0 1 7.16
CHEMBL1323 DARUNAVIR 4.0 1 6.70
CHEMBL729 LOPINAVIR 4.0 1 6.41
CHEMBL116 AMPRENAVIR 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4214453 EC50 = 0.3 nM 9.52
CHEMBL4207145 EC50 = 0.32 nM 9.49
CHEMBL1163 ATAZANAVIR EC50 = 70.0 nM 7.16
CHEMBL1323 DARUNAVIR EC50 = 200.0 nM 6.70
CHEMBL729 LOPINAVIR EC50 = 390.0 nM 6.41
CHEMBL116 AMPRENAVIR EC50 > 1000.0 nM -