Assay Detail
Binding
CHEMBL4196285
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of His6-tagged EGFR (unknown origin) cytoplasmic domain expressed in baculovirus infected Sf9 insect cells after 10 mins followed by addition of ATP-MgCl2 and measured after 1 hr by time-resolved fluorometric analysis
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Sf9 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Design, synthesis, mechanistic and histopathological studies of small-molecules of novel indole-2-carboxamides and pyrazino[1,2-a]indol-1(2H)-ones as potential anticancer agents effecting the reactive oxygen species production.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 7 | 6.05 | 7.10 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL553 | ERLOTINIB | 4.0 | 1 | 7.10 |
| CHEMBL4209588 | — | — | 1 | 6.30 |
| CHEMBL4214496 | — | — | 1 | 6.10 |
| CHEMBL4207316 | — | — | 1 | 6.05 |
| CHEMBL4208224 | — | — | 1 | 5.77 |
| CHEMBL4208745 | — | — | 1 | 5.62 |
| CHEMBL4213146 | — | — | 1 | 5.41 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL553 | ERLOTINIB | IC50 | = | 80.0 | nM | 7.10 |
| CHEMBL4209588 | — | IC50 | = | 500.0 | nM | 6.30 |
| CHEMBL4214496 | — | IC50 | = | 800.0 | nM | 6.10 |
| CHEMBL4207316 | — | IC50 | = | 900.0 | nM | 6.05 |
| CHEMBL4208224 | — | IC50 | = | 1700.0 | nM | 5.77 |
| CHEMBL4208745 | — | IC50 | = | 2400.0 | nM | 5.62 |
| CHEMBL4213146 | — | IC50 | = | 3900.0 | nM | 5.41 |