Assay Detail
Binding
CHEMBL4197608
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Inhibition of Escherichia coli BL21(DE3) Leucyl-tRNA synthetase assessed as reduction in tRNA aminoacylation preincubated for 10 mins with Escherichia coli tRNA and 14C-labeled leucine followed by ATP addition by liquid scintillation counting method
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Escherichia coli BL21(DE3) |
| Confidence | 8 — Homologous single protein target |
| Curated By | Intermediate |
Target
Leucine--tRNA ligase (CHEMBL4295566) ↗| Type | SINGLE PROTEIN |
| Organism | Escherichia coli (strain K12) |
Publication
Family-wide analysis of aminoacyl-sulfamoyl-3-deazaadenosine analogues as inhibitors of aminoacyl-tRNA synthetases.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 2 | 9.52 | 9.86 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1163081 | LEUSA | — | 1 | 9.86 |
| CHEMBL4204314 | — | — | 1 | 9.18 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1163081 | LEUSA | Ki | = | 0.139 | nM | 9.86 |
| CHEMBL4204314 | — | Ki | = | 0.654 | nM | 9.18 |