Assay Detail
Binding
CHEMBL4197842
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of porcupine in mouse L Wnt3A cells co-cultured with HEK293 cells assessed as suppression of Wnt signaling after 48 hrs by Super-top flash reporter gene assay
6
Total Activities
6
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Mus musculus |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Protein-serine O-palmitoleoyltransferase porcupine (CHEMBL1255164) ↗| Type | SINGLE PROTEIN |
| Organism | Mus musculus |
Publication
Discovery and characterization of a potent Wnt and hedgehog signaling pathways dual inhibitor.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 5 | 8.76 | 9.14 |
| Inhibition | 1 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4215992 | — | — | 1 | 9.14 |
| CHEMBL3188386 | WNT-974 | 2.0 | 1 | 9.05 |
| CHEMBL4215124 | — | — | 1 | 8.72 |
| CHEMBL3923788 | — | — | 1 | 8.68 |
| CHEMBL3910011 | — | — | 1 | 8.19 |
| CHEMBL473417 | VISMODEGIB | 4.0 | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4215992 | — | IC50 | = | 0.72 | nM | 9.14 |
| CHEMBL3188386 | WNT-974 | IC50 | = | 0.9 | nM | 9.05 |
| CHEMBL4215124 | — | IC50 | = | 1.9 | nM | 8.72 |
| CHEMBL3923788 | — | IC50 | = | 2.1 | nM | 8.68 |
| CHEMBL3910011 | — | IC50 | = | 6.5 | nM | 8.19 |
| CHEMBL473417 | VISMODEGIB | Inhibition | - | % | - |