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Assay Detail

CHEMBL4198913

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of full length human recombinant N-terminal GST-tagged PDE5A1 expressed in baculovirus infected sf9 cells using cGMP as substrate after 30 mins by fluorescence assay
25
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

cGMP-specific 3',5'-cyclic phosphodiesterase (CHEMBL1827)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis, biological evaluation and in vivo testing of dual phosphodiesterase 5 (PDE5) and histone deacetylase 6 (HDAC6)-selective inhibitors for the treatment of Alzheimer's disease.
Rabal O, Sánchez-Arias JA, Cuadrado-Tejedor M, de Miguel I, Pérez-González M, García-Barroso C, Ugarte A, Estella-Hermoso de Mendoza A, Sáez E, Espelosin M, Ursua S, Haizhong T, Wei W, Musheng X, Garcia-Osta A, Oyarzabal J.
Eur J Med Chem (2018) 150 : 506 -524
PubMed 29549837 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 25 7.76 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4216635 1 9.00
CHEMBL4209220 1 8.70
CHEMBL4203481 1 8.70
CHEMBL4217166 1 8.52
CHEMBL4214135 1 8.52
CHEMBL4214699 1 8.52
CHEMBL4202521 1 8.15
CHEMBL4212445 1 8.00
CHEMBL4204952 1 8.00
CHEMBL4212186 1 7.96
CHEMBL4213615 1 7.89
CHEMBL4210653 1 7.85
CHEMBL4212866 1 7.82
CHEMBL4207538 1 7.70
CHEMBL4207409 1 7.57
CHEMBL4209025 1 7.51
CHEMBL4218554 1 7.44
CHEMBL4216348 1 7.33
CHEMBL4217821 1 7.32
CHEMBL4206296 1 7.28
CHEMBL4207836 1 7.10
CHEMBL4205788 1 6.84
CHEMBL4204679 1 6.84
CHEMBL4204146 1 6.83
CHEMBL4217999 1 6.72

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4216635 IC50 = 1.0 nM 9.00
CHEMBL4209220 IC50 = 2.0 nM 8.70
CHEMBL4203481 IC50 = 2.0 nM 8.70
CHEMBL4217166 IC50 = 3.0 nM 8.52
CHEMBL4214135 IC50 = 3.0 nM 8.52
CHEMBL4214699 IC50 = 3.0 nM 8.52
CHEMBL4202521 IC50 = 7.0 nM 8.15
CHEMBL4212445 IC50 = 10.0 nM 8.00
CHEMBL4204952 IC50 = 10.0 nM 8.00
CHEMBL4212186 IC50 = 11.0 nM 7.96
CHEMBL4213615 IC50 = 13.0 nM 7.89
CHEMBL4210653 IC50 = 14.0 nM 7.85
CHEMBL4212866 IC50 = 15.0 nM 7.82
CHEMBL4207538 IC50 = 20.0 nM 7.70
CHEMBL4207409 IC50 = 27.0 nM 7.57
CHEMBL4209025 IC50 = 31.0 nM 7.51
CHEMBL4218554 IC50 = 36.0 nM 7.44
CHEMBL4216348 IC50 = 47.0 nM 7.33
CHEMBL4217821 IC50 = 48.0 nM 7.32
CHEMBL4206296 IC50 = 53.0 nM 7.28
CHEMBL4207836 IC50 = 79.0 nM 7.10
CHEMBL4205788 IC50 = 146.0 nM 6.84
CHEMBL4204679 IC50 = 144.0 nM 6.84
CHEMBL4204146 IC50 = 149.0 nM 6.83
CHEMBL4217999 IC50 = 190.0 nM 6.72