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Assay Detail

CHEMBL4200456

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of ATM autophosphorylation at Ser1981 residue in human HT-29 cells measured after 1 hr by Hoechst staining based fluorescence assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HT-29
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine-protein kinase ATM (CHEMBL3797)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a Series of 3-Cinnoline Carboxamides as Orally Bioavailable, Highly Potent, and Selective ATM Inhibitors.
Barlaam B, Cadogan E, Campbell A, Colclough N, Dishington A, Durant S, Goldberg K, Hassall LA, Hughes GD, MacFaul PA, McGuire TM, Pass M, Patel A, Pearson S, Petersen J, Pike KG, Robb G, Stratton N, Xin G, Zhai B.
ACS Med Chem Lett (2018) 9 : 809 -814
PubMed 30128072 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 7.90 9.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4210045 1 9.30
CHEMBL4212961 1 9.05
CHEMBL4208569 1 8.96
CHEMBL4213503 1 8.74
CHEMBL4217549 1 8.72
CHEMBL4218854 1 8.55
CHEMBL4213919 1 8.41
CHEMBL4217902 1 8.41
CHEMBL4215266 1 8.00
CHEMBL4166405 1 7.48
CHEMBL4205148 1 7.46
CHEMBL4216325 1 7.36
CHEMBL4218734 1 7.34
CHEMBL4205675 1 7.31
CHEMBL4217379 1 7.21
CHEMBL4209427 1 6.85
CHEMBL4210849 1 6.58
CHEMBL4205554 1 6.48

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4210045 IC50 = 0.5 nM 9.30
CHEMBL4212961 IC50 = 0.9 nM 9.05
CHEMBL4208569 IC50 = 1.1 nM 8.96
CHEMBL4213503 IC50 = 1.8 nM 8.74
CHEMBL4217549 IC50 = 1.9 nM 8.72
CHEMBL4218854 IC50 = 2.8 nM 8.55
CHEMBL4213919 IC50 = 3.9 nM 8.41
CHEMBL4217902 IC50 = 3.9 nM 8.41
CHEMBL4215266 IC50 = 10.0 nM 8.00
CHEMBL4166405 IC50 = 33.0 nM 7.48
CHEMBL4205148 IC50 = 35.0 nM 7.46
CHEMBL4216325 IC50 = 44.0 nM 7.36
CHEMBL4218734 IC50 = 46.0 nM 7.34
CHEMBL4205675 IC50 = 49.0 nM 7.31
CHEMBL4217379 IC50 = 61.0 nM 7.21
CHEMBL4209427 IC50 = 140.0 nM 6.85
CHEMBL4210849 IC50 = 260.0 nM 6.58
CHEMBL4205554 IC50 = 330.0 nM 6.48