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Assay Detail

CHEMBL4202175

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at rat alpha3beta4 nACHR expressed in Xenopus laevis oocytes assessed as inhibition of acetyl choline-induced membrane currents pre-incubated for 5 mins prior to acetyl choline pulse at -70 mV potential by two-electrode voltage clamp assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 7 — Homologous single protein target
Curated By Intermediate

Target

Neuronal acetylcholine receptor; alpha3/beta4 (CHEMBL1907593)
Type PROTEIN COMPLEX
Organism Rattus norvegicus

Publication

Single Amino Acid Substitution in α-Conotoxin TxID Reveals a Specific α3β4 Nicotinic Acetylcholine Receptor Antagonist.
Yu J, Zhu X, Harvey PJ, Kaas Q, Zhangsun D, Craik DJ, Luo S.
J Med Chem (2018) 61 : 9256 -9265
PubMed 30252466 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 7.86 8.72

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4218406 1 8.72
CHEMBL4214931 1 8.70
CHEMBL3104245 1 8.44
CHEMBL4214003 1 8.41
CHEMBL4210514 1 8.27
CHEMBL4215824 1 8.16
CHEMBL4206670 1 8.13
CHEMBL4215453 1 8.10
CHEMBL4211045 1 8.00
CHEMBL4206157 1 7.96
CHEMBL4208491 1 7.28
CHEMBL4203617 1 6.92
CHEMBL4204149 1 6.50
CHEMBL4203278 1 6.42
CHEMBL4207977 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4218406 IC50 = 1.9 nM 8.72
CHEMBL4214931 IC50 = 2.0 nM 8.70
CHEMBL3104245 IC50 = 3.6 nM 8.44
CHEMBL4214003 IC50 = 3.9 nM 8.41
CHEMBL4210514 IC50 = 5.4 nM 8.27
CHEMBL4215824 IC50 = 6.9 nM 8.16
CHEMBL4206670 IC50 = 7.4 nM 8.13
CHEMBL4215453 IC50 = 7.9 nM 8.10
CHEMBL4211045 IC50 = 9.9 nM 8.00
CHEMBL4206157 IC50 = 11.0 nM 7.96
CHEMBL4208491 IC50 = 52.0 nM 7.28
CHEMBL4203617 IC50 = 120.0 nM 6.92
CHEMBL4204149 IC50 = 320.0 nM 6.50
CHEMBL4203278 IC50 = 380.0 nM 6.42
CHEMBL4207977 IC50 > 10000.0 nM -