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Assay Detail

CHEMBL4222846

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of EGFR L858R/T790M double mutant (unknown origin) using poly (Glu, Tyr) as substrate after 40 mins by kinase-glo plus luminescence assay
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and evaluation of 2,9-disubstituted 8-phenylthio/phenylsulfinyl-9H-purine as new EGFR inhibitors.
Hei YY, Shen Y, Wang J, Zhang H, Zhao HY, Xin M, Cao YX, Li Y, Zhang SQ.
Bioorg Med Chem (2018) 26 : 2173 -2185
PubMed 29576272 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 7.48 8.74

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3353410 OSIMERTINIB 4.0 1 8.74
CHEMBL4226676 1 6.98
CHEMBL4228518 1 6.72
CHEMBL4227084 1 -
CHEMBL4225951 1 -
CHEMBL4226937 1 -
CHEMBL4225777 1 -
CHEMBL4225565 1 -
CHEMBL4226151 1 -
CHEMBL4225306 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3353410 OSIMERTINIB IC50 = 1.8 nM 8.74
CHEMBL4226676 IC50 = 104.0 nM 6.98
CHEMBL4228518 IC50 = 189.0 nM 6.72
CHEMBL4227084 IC50 - -
CHEMBL4225951 IC50 - -
CHEMBL4226937 IC50 - -
CHEMBL4225777 IC50 - -
CHEMBL4225565 IC50 - -
CHEMBL4226151 IC50 - -
CHEMBL4225306 IC50 - -