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Assay Detail

CHEMBL4223293

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of KDM5A/KDM5B in human ZR-75-1 cells assessed as increase in H3K4me3 level after 72 hrs by fluorometric immunoassay
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type ZR-75-1
Confidence 5 — Multiple protein complex / RNA
Curated By Intermediate

Target

Lysine-specific demethylase 5A/5B (CHEMBL4296073)
Type PROTEIN FAMILY
Organism Homo sapiens

Publication

Structure-based design and discovery of potent and selective KDM5 inhibitors.
Nie Z, Shi L, Lai C, O'Connell SM, Xu J, Stansfield RK, Hosfield DJ, Veal JM, Stafford JA.
Bioorg Med Chem Lett (2018) 28 : 1490 -1494
PubMed 29627262 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 14 5.99 7.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3786952 1 7.05
CHEMBL4228292 1 6.96
CHEMBL3785832 1 6.38
CHEMBL4227526 1 6.10
CHEMBL4225013 1 6.08
CHEMBL4226978 1 6.07
CHEMBL4229271 1 6.03
CHEMBL4228087 1 6.00
CHEMBL4229264 1 6.00
CHEMBL4227237 1 5.89
CHEMBL4228546 1 5.89
CHEMBL4228806 1 5.34
CHEMBL4225358 1 5.16
CHEMBL4228781 1 4.92

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3786952 EC50 = 90.0 nM 7.05
CHEMBL4228292 EC50 = 110.0 nM 6.96
CHEMBL3785832 EC50 = 420.0 nM 6.38
CHEMBL4227526 EC50 = 800.0 nM 6.10
CHEMBL4225013 EC50 = 840.0 nM 6.08
CHEMBL4226978 EC50 = 860.0 nM 6.07
CHEMBL4229271 EC50 = 940.0 nM 6.03
CHEMBL4228087 EC50 = 990.0 nM 6.00
CHEMBL4229264 EC50 = 1000.0 nM 6.00
CHEMBL4227237 EC50 = 1300.0 nM 5.89
CHEMBL4228546 EC50 = 1300.0 nM 5.89
CHEMBL4228806 EC50 = 4600.0 nM 5.34
CHEMBL4225358 EC50 = 7000.0 nM 5.16
CHEMBL4228781 EC50 = 12000.0 nM 4.92