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Assay Detail

CHEMBL4223545

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Plasmodium falciparum plasmepsin-2 using DABCYL-Glu-Arg-Nle-Phe-Leu-Ser-Phe-Pro-EDANS as substrate preincubated for 30 mins followed by substrate addition measured every 1 min within 8 to 15 mins by FRET assay
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Plasmodium falciparum
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Plasmepsin II (CHEMBL4414)
Type SINGLE PROTEIN
Organism Plasmodium falciparum

Publication

2-Aminoquinazolin-4(3H)-one based plasmepsin inhibitors with improved hydrophilicity and selectivity.
Rasina D, Stakanovs G, Borysov OV, Pantelejevs T, Bobrovs R, Kanepe-Lapsa I, Tars K, Jaudzems K, Jirgensons A.
Bioorg Med Chem (2018) 26 : 2488 -2500
PubMed 29636223 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 6.73 9.38

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL296588 PEPSTATIN 2.0 1 9.38
CHEMBL4225298 1 7.66
CHEMBL4227074 1 7.64
CHEMBL4225824 1 7.57
CHEMBL4228456 1 7.12
CHEMBL4228072 1 7.10
CHEMBL4225299 1 7.06
CHEMBL4226087 1 7.03
CHEMBL3764174 1 6.82
CHEMBL4226891 1 6.55
CHEMBL4225038 1 6.48
CHEMBL4224896 1 6.40
CHEMBL4227924 1 6.32
CHEMBL4229013 1 6.30
CHEMBL4225481 1 6.30
CHEMBL4229080 1 6.22
CHEMBL4225394 1 6.16
CHEMBL4228633 1 6.09
CHEMBL4226144 1 6.05
CHEMBL4228400 1 6.00
CHEMBL3763523 1 5.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL296588 PEPSTATIN IC50 = 0.42 nM 9.38
CHEMBL4225298 IC50 = 22.0 nM 7.66
CHEMBL4227074 IC50 = 23.0 nM 7.64
CHEMBL4225824 IC50 = 27.0 nM 7.57
CHEMBL4228456 IC50 = 75.0 nM 7.12
CHEMBL4228072 IC50 = 80.0 nM 7.10
CHEMBL4225299 IC50 = 88.0 nM 7.06
CHEMBL4226087 IC50 = 93.0 nM 7.03
CHEMBL3764174 IC50 = 150.0 nM 6.82
CHEMBL4226891 IC50 = 280.0 nM 6.55
CHEMBL4225038 IC50 = 330.0 nM 6.48
CHEMBL4224896 IC50 = 400.0 nM 6.40
CHEMBL4227924 IC50 = 480.0 nM 6.32
CHEMBL4229013 IC50 = 500.0 nM 6.30
CHEMBL4225481 IC50 = 500.0 nM 6.30
CHEMBL4229080 IC50 = 600.0 nM 6.22
CHEMBL4225394 IC50 = 700.0 nM 6.16
CHEMBL4228633 IC50 = 820.0 nM 6.09
CHEMBL4226144 IC50 = 900.0 nM 6.05
CHEMBL4228400 IC50 = 1000.0 nM 6.00
CHEMBL3763523 IC50 = 10000.0 nM 5.00