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Assay Detail

CHEMBL4223792

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Reversible inhibition of human TGase 2 using Z-Glu(HMC)-Gly-OH as substrate measured at 20 secs interval over 900 secs by fluorimetric assay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Protein-glutamine gamma-glutamyltransferase (CHEMBL2730)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Nε-Acryloyllysine Piperazides as Irreversible Inhibitors of Transglutaminase 2: Synthesis, Structure-Activity Relationships, and Pharmacokinetic Profiling.
Wodtke R, Hauser C, Ruiz-Gómez G, Jäckel E, Bauer D, Lohse M, Wong A, Pufe J, Ludwig FA, Fischer S, Hauser S, Greif D, Pisabarro MT, Pietzsch J, Pietsch M, Löser R.
J Med Chem (2018) 61 : 4528 -4560
PubMed 29664627 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 12 5.17 5.65

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4227301 1 5.65
CHEMBL4227835 1 5.54
CHEMBL4228542 1 5.46
CHEMBL4227328 1 5.35
CHEMBL4228671 1 5.25
CHEMBL2203451 1 5.24
CHEMBL4228168 1 5.20
CHEMBL4228146 1 5.13
CHEMBL4226785 1 5.11
CHEMBL4225490 1 5.08
CHEMBL4229246 1 4.82
CHEMBL4227629 1 4.19

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4227301 Ki = 2260.0 nM 5.65
CHEMBL4227835 Ki = 2920.0 nM 5.54
CHEMBL4228542 Ki = 3500.0 nM 5.46
CHEMBL4227328 Ki = 4430.0 nM 5.35
CHEMBL4228671 Ki = 5650.0 nM 5.25
CHEMBL2203451 Ki = 5730.0 nM 5.24
CHEMBL4228168 Ki = 6280.0 nM 5.20
CHEMBL4228146 Ki = 7320.0 nM 5.13
CHEMBL4226785 Ki = 7760.0 nM 5.11
CHEMBL4225490 Ki = 8420.0 nM 5.08
CHEMBL4229246 Ki = 15000.0 nM 4.82
CHEMBL4227629 Ki = 64300.0 nM 4.19