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Assay Detail

CHEMBL4224043

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Plasmodium falciparum 3D7 FLN (55 to 1193 residues) expressed in Escherichia coli BL21 using Dabcyl-HKRHSFRMRG-EDANS as substrate preincubated for 5 mins followed by substrate addition measured for 10 mins by fluorescence assay
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Plasmodium falciparum 3D7
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Falcilysin (CHEMBL4295876)
Type SINGLE PROTEIN
Organism Plasmodium falciparum (isolate 3D7)

Publication

Development of piperazine-based hydroxamic acid inhibitors against falcilysin, an essential malarial protease.
Chance JP, Fejzic H, Hernandez O, Istvan ES, Andaya A, Maslov N, Aispuro R, Crisanto T, Nguyen H, Vidal B, Serrano W, Kuwahara B, Pugne Andanado C, Goldberg DE, Mallari JP.
Bioorg Med Chem Lett (2018) 28 : 1846 -1848
PubMed 29691139 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 4.94 5.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4225532 1 5.82
CHEMBL4226440 1 5.30
CHEMBL4228497 1 5.22
CHEMBL4227329 1 5.16
CHEMBL4227286 1 5.10
CHEMBL4228556 1 5.10
CHEMBL125598 1 5.00
CHEMBL4227719 1 5.00
CHEMBL4227787 1 4.89
CHEMBL4224730 1 4.85
CHEMBL4225559 1 4.80
CHEMBL4225657 1 4.52
CHEMBL4229016 1 4.51
CHEMBL4225259 1 4.46
CHEMBL4226498 1 4.40
CHEMBL421644 1 -
CHEMBL4228814 1 -
CHEMBL4225711 1 -
CHEMBL4225755 1 -
CHEMBL4228238 1 -
CHEMBL4226235 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4225532 IC50 = 1500.0 nM 5.82
CHEMBL4226440 IC50 = 5000.0 nM 5.30
CHEMBL4228497 IC50 = 6000.0 nM 5.22
CHEMBL4227329 IC50 = 7000.0 nM 5.16
CHEMBL4227286 IC50 = 8000.0 nM 5.10
CHEMBL4228556 IC50 = 8000.0 nM 5.10
CHEMBL125598 IC50 = 10000.0 nM 5.00
CHEMBL4227719 IC50 = 10000.0 nM 5.00
CHEMBL4227787 IC50 = 13000.0 nM 4.89
CHEMBL4224730 IC50 = 14000.0 nM 4.85
CHEMBL4225559 IC50 = 16000.0 nM 4.80
CHEMBL4225657 IC50 = 30000.0 nM 4.52
CHEMBL4229016 IC50 = 31000.0 nM 4.51
CHEMBL4225259 IC50 = 35000.0 nM 4.46
CHEMBL4226498 IC50 = 40000.0 nM 4.40
CHEMBL421644 IC50 > 50000.0 nM -
CHEMBL4228814 IC50 > 50000.0 nM -
CHEMBL4225711 IC50 > 50000.0 nM -
CHEMBL4225755 IC50 > 50000.0 nM -
CHEMBL4228238 IC50 > 50000.0 nM -
CHEMBL4226235 IC50 > 50000.0 nM -