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Assay Detail

CHEMBL4235697

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of BTK in human Ramos cells assessed as reduction in intracellular calcium level incubated for 1 hr measured for 180 secs by FLIPR assay
34
Total Activities
34
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Ramos
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase BTK (CHEMBL5251)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Conversion of carbazole carboxamide based reversible inhibitors of Bruton's tyrosine kinase (BTK) into potent, selective irreversible inhibitors in the carbazole, tetrahydrocarbazole, and a new 2,3-dimethylindole series.
Liu Q, Batt DG, Chaudhry C, Lippy JS, Pattoli MA, Surti N, Xu S, Carter PH, Burke JR, Tino JA.
Bioorg Med Chem Lett (2018) 28 : 3080 -3084
PubMed 30097367 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 34 7.27 8.34

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4241958 1 8.34
CHEMBL4248719 1 8.29
CHEMBL4242527 1 8.19
CHEMBL4239747 1 8.15
CHEMBL4248550 1 8.06
CHEMBL4237557 1 8.04
CHEMBL4239031 1 7.82
CHEMBL4245037 1 7.82
CHEMBL4243995 1 7.80
CHEMBL4248386 1 7.64
CHEMBL4248271 1 7.64
CHEMBL3918580 1 7.58
CHEMBL4246324 1 7.58
CHEMBL4237249 1 7.55
CHEMBL4238798 1 7.47
CHEMBL4248834 1 7.24
CHEMBL4238256 1 7.23
CHEMBL4242058 1 7.21
CHEMBL4237999 1 7.20
CHEMBL4246005 1 7.17
CHEMBL4251151 1 7.12
CHEMBL4237800 1 6.92
CHEMBL4247992 1 6.77
CHEMBL4249247 1 6.70
CHEMBL4244928 1 6.54
CHEMBL4240731 1 6.43
CHEMBL4241723 1 6.05
CHEMBL4238297 1 6.00
CHEMBL4243299 1 5.77
CHEMBL4238212 1 5.70

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4241958 IC50 = 4.6 nM 8.34
CHEMBL4248719 IC50 = 5.1 nM 8.29
CHEMBL4242527 IC50 = 6.4 nM 8.19
CHEMBL4239747 IC50 = 7.1 nM 8.15
CHEMBL4248550 IC50 = 8.8 nM 8.06
CHEMBL4237557 IC50 = 9.2 nM 8.04
CHEMBL4239031 IC50 = 15.0 nM 7.82
CHEMBL4245037 IC50 = 15.0 nM 7.82
CHEMBL4243995 IC50 = 16.0 nM 7.80
CHEMBL4248386 IC50 = 23.0 nM 7.64
CHEMBL4248271 IC50 = 23.0 nM 7.64
CHEMBL3918580 IC50 = 26.0 nM 7.58
CHEMBL4246324 IC50 = 26.0 nM 7.58
CHEMBL4237249 IC50 = 28.0 nM 7.55
CHEMBL4238798 IC50 = 34.0 nM 7.47
CHEMBL4248834 IC50 = 57.0 nM 7.24
CHEMBL4238256 IC50 = 59.0 nM 7.23
CHEMBL4242058 IC50 = 61.0 nM 7.21
CHEMBL4237999 IC50 = 63.0 nM 7.20
CHEMBL4246005 IC50 = 68.0 nM 7.17
CHEMBL4251151 IC50 = 75.0 nM 7.12
CHEMBL4237800 IC50 = 120.0 nM 6.92
CHEMBL4247992 IC50 = 170.0 nM 6.77
CHEMBL4249247 IC50 = 200.0 nM 6.70
CHEMBL4244928 IC50 = 290.0 nM 6.54
CHEMBL4240731 IC50 = 370.0 nM 6.43
CHEMBL4241723 IC50 = 890.0 nM 6.05
CHEMBL4238297 IC50 = 1000.0 nM 6.00
CHEMBL4243299 IC50 = 1700.0 nM 5.77
CHEMBL4238212 IC50 = 2000.0 nM 5.70
CHEMBL4240879 IC50 - -
CHEMBL4245876 IC50 - -
CHEMBL4237436 IC50 > 2000.0 nM -
CHEMBL4249677 IC50 > 2000.0 nM -