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Assay Detail

CHEMBL4236252

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Binding
Inhibition of human full length BTK using FITC-AHA-EEPLYWSFPAKKK-NH2 as substrate after 90 mins by microfluid mobility shift assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase BTK (CHEMBL5251)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of potent, highly selective covalent irreversible BTK inhibitors from a fragment hit.
Qiu H, Liu-Bujalski L, Caldwell RD, Follis AV, Gardberg A, Goutopoulos A, Grenningloh R, Head J, Johnson T, Jones R, Mochalkin I, Morandi F, Neagu C, Sherer B.
Bioorg Med Chem Lett (2018) 28 : 2939 -2944
PubMed 30122225 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 7.77 9.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1873475 IBRUTINIB 4.0 1 9.70
CHEMBL4242598 1 9.15
CHEMBL4250200 1 8.39
CHEMBL4246399 1 8.36
CHEMBL4246409 1 7.92
CHEMBL4243203 1 7.89
CHEMBL4241920 1 7.60
CHEMBL4240087 1 7.50
CHEMBL4238999 1 7.40
CHEMBL4239634 1 6.37
CHEMBL4243865 1 5.23

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1873475 IBRUTINIB IC50 = 0.2 nM 9.70
CHEMBL4242598 IC50 = 0.7 nM 9.15
CHEMBL4250200 IC50 = 4.1 nM 8.39
CHEMBL4246399 IC50 = 4.4 nM 8.36
CHEMBL4246409 IC50 = 12.0 nM 7.92
CHEMBL4243203 IC50 = 13.0 nM 7.89
CHEMBL4241920 IC50 = 25.0 nM 7.60
CHEMBL4240087 IC50 = 32.0 nM 7.50
CHEMBL4238999 IC50 = 40.0 nM 7.40
CHEMBL4239634 IC50 = 430.0 nM 6.37
CHEMBL4243865 IC50 = 5900.0 nM 5.23