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Assay Detail

CHEMBL4252030

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of porcine pancreatic alpha-amylase using starch as substrate preincubated for 10 mins followed by substrate addition measured after 10 mins by dinitrosalicylic acid color reagent based assay
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Sus scrofa
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Pancreatic alpha-amylase (CHEMBL5730)
Type SINGLE PROTEIN
Organism Sus scrofa

Publication

Synthesis of piperazine sulfonamide analogs as diabetic-II inhibitors and their molecular docking study.
Taha M, Irshad M, Imran S, Chigurupati S, Selvaraj M, Rahim F, Ismail NH, Nawaz F, Khan KM.
Eur J Med Chem (2017) 141 : 530 -537
PubMed 29102178 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 5.56 5.87

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL404271 ACARBOSE 4.0 1 5.87
CHEMBL4277658 1 5.80
CHEMBL4284909 1 5.68
CHEMBL4294256 1 5.67
CHEMBL4292777 1 5.63
CHEMBL4290115 1 5.57
CHEMBL4286367 1 5.56
CHEMBL4288298 1 5.55
CHEMBL4283335 1 5.54
CHEMBL4291709 1 5.54
CHEMBL4278776 1 5.54
CHEMBL4293927 1 5.53
CHEMBL4279414 1 5.53
CHEMBL4283744 1 5.51
CHEMBL4286685 1 5.50
CHEMBL4285663 1 5.50
CHEMBL4293503 1 5.46
CHEMBL4282249 1 5.45
CHEMBL4291606 1 5.45
CHEMBL4281074 1 5.40

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL404271 ACARBOSE IC50 = 1353.0 nM 5.87
CHEMBL4277658 IC50 = 1571.0 nM 5.80
CHEMBL4284909 IC50 = 2064.0 nM 5.68
CHEMBL4294256 IC50 = 2118.0 nM 5.67
CHEMBL4292777 IC50 = 2348.0 nM 5.63
CHEMBL4290115 IC50 = 2670.0 nM 5.57
CHEMBL4286367 IC50 = 2751.0 nM 5.56
CHEMBL4288298 IC50 = 2823.0 nM 5.55
CHEMBL4283335 IC50 = 2881.0 nM 5.54
CHEMBL4291709 IC50 = 2874.0 nM 5.54
CHEMBL4278776 IC50 = 2883.0 nM 5.54
CHEMBL4293927 IC50 = 2981.0 nM 5.53
CHEMBL4279414 IC50 = 2927.0 nM 5.53
CHEMBL4283744 IC50 = 3052.0 nM 5.51
CHEMBL4286685 IC50 = 3140.0 nM 5.50
CHEMBL4285663 IC50 = 3199.0 nM 5.50
CHEMBL4293503 IC50 = 3448.0 nM 5.46
CHEMBL4282249 IC50 = 3524.0 nM 5.45
CHEMBL4291606 IC50 = 3560.0 nM 5.45
CHEMBL4281074 IC50 = 3980.0 nM 5.40