Assay Detail
Binding
CHEMBL4253025
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Inhibition of bovine milk xanthine oxidase using xanthine as substrate preincubated for 15 mins followed by substrate addition measured after 30 mins by UV-VIS spectrophotometric analysis
19
Total Activities
10
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Bos taurus |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Xanthine oxidase inhibitory activity of natural and hemisynthetic flavonoids from Gardenia oudiepe (Rubiaceae) in vitro and molecular docking studies.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 10 | 6.10 | 8.40 |
| Inhibition | 9 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL165064 | ISOKAEMPFERIDE | — | 2 | 8.40 |
| CHEMBL351607 | 3,6-DIMETHOXY KAEMPFEROL | — | 2 | 7.30 |
| CHEMBL1689269 | — | — | 2 | 7.05 |
| CHEMBL161957 | SANTIN | — | 2 | 6.66 |
| CHEMBL1467 | ALLOPURINOL | 4.0 | 1 | 6.60 |
| CHEMBL3407503 | — | — | 2 | 5.34 |
| CHEMBL3407749 | — | — | 2 | 5.16 |
| CHEMBL77686 | — | — | 2 | 5.01 |
| CHEMBL521934 | — | — | 2 | 4.83 |
| CHEMBL4294702 | — | — | 2 | 4.68 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL165064 | ISOKAEMPFERIDE | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL351607 | 3,6-DIMETHOXY KAEMPFEROL | IC50 | = | 50.0 | nM | 7.30 |
| CHEMBL1689269 | — | IC50 | = | 90.0 | nM | 7.05 |
| CHEMBL161957 | SANTIN | IC50 | = | 220.0 | nM | 6.66 |
| CHEMBL1467 | ALLOPURINOL | IC50 | = | 250.0 | nM | 6.60 |
| CHEMBL3407503 | — | IC50 | = | 4540.0 | nM | 5.34 |
| CHEMBL3407749 | — | IC50 | = | 6950.0 | nM | 5.16 |
| CHEMBL77686 | — | IC50 | = | 9790.0 | nM | 5.01 |
| CHEMBL521934 | — | IC50 | = | 14900.0 | nM | 4.83 |
| CHEMBL4294702 | — | IC50 | = | 21030.0 | nM | 4.68 |
| CHEMBL3407749 | — | Inhibition | - | % | - | |
| CHEMBL3407503 | — | Inhibition | - | % | - | |
| CHEMBL161957 | SANTIN | Inhibition | - | % | - | |
| CHEMBL1689269 | — | Inhibition | - | % | - | |
| CHEMBL351607 | 3,6-DIMETHOXY KAEMPFEROL | Inhibition | - | % | - | |
| CHEMBL165064 | ISOKAEMPFERIDE | Inhibition | - | % | - | |
| CHEMBL77686 | — | Inhibition | - | % | - | |
| CHEMBL521934 | — | Inhibition | - | % | - | |
| CHEMBL4294702 | — | Inhibition | - | % | - |