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Assay Detail

CHEMBL4253928

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Non-competitive inhibition of human PTP1B using p-nitrophenyl phosphate as substrate up to 7 uM preincubated for 10 mins followed by substrate addition measured every minute for 10 mins by Michaelis-Menten plot analysis
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein phosphatase non-receptor type 1 (CHEMBL335)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Naturally occurring Diels-Alder-type adducts from Morus nigra as potent inhibitors of Mycobacterium tuberculosis protein tyrosine phosphatase B.
Mascarello A, Orbem Menegatti AC, Calcaterra A, Martins PGA, Chiaradia-Delatorre LD, D'Acquarica I, Ferrari F, Pau V, Sanna A, De Logu A, Botta M, Botta B, Terenzi H, Mori M.
Eur J Med Chem (2018) 144 : 277 -288
PubMed 29275228 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 1 6.70 6.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4279417 1 6.70

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4279417 Ki = 200.0 nM 6.70