Assay Detail
Functional
CHEMBL4255281
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Agonist activity at N-terminal HA-tagged human CB1 receptor expressed in HEK293 cells transfected with YFP-Epac-RLuc assessed as decrease in forskolin-stimulated cAMP level by BRET assay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | HEK293 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Alkyl indole-based cannabinoid type 2 receptor tools: Exploration of linker and fluorophore attachment.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 12 | 6.70 | 7.20 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4284943 | — | — | 1 | 7.20 |
| CHEMBL4282604 | — | — | 1 | 6.20 |
| CHEMBL4277575 | — | — | 1 | - |
| CHEMBL78627 | — | — | 1 | - |
| CHEMBL4292807 | — | — | 1 | - |
| CHEMBL4293194 | — | — | 1 | - |
| CHEMBL4295181 | — | — | 1 | - |
| CHEMBL4279405 | — | — | 1 | - |
| CHEMBL4283967 | — | — | 1 | - |
| CHEMBL4291156 | — | — | 1 | - |
| CHEMBL4294653 | — | — | 1 | - |
| CHEMBL559612 | CP-55940 | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4284943 | — | EC50 | = | 62.9 | nM | 7.20 |
| CHEMBL4282604 | — | EC50 | = | 632.5 | nM | 6.20 |
| CHEMBL4277575 | — | EC50 | - | — | - | |
| CHEMBL78627 | — | EC50 | - | — | - | |
| CHEMBL4292807 | — | EC50 | - | — | - | |
| CHEMBL4293194 | — | EC50 | - | — | - | |
| CHEMBL4295181 | — | EC50 | - | — | - | |
| CHEMBL4279405 | — | EC50 | - | — | - | |
| CHEMBL4283967 | — | EC50 | - | — | - | |
| CHEMBL4291156 | — | EC50 | - | — | - | |
| CHEMBL4294653 | — | EC50 | - | — | - | |
| CHEMBL559612 | CP-55940 | EC50 | - | — | - |