Assay Detail
Binding
CHEMBL4256087
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of Aquifex aeolicus MraY expressed in Escherichia coli C41(DE3) using Park's nucleotide as substrate pretreated for 30 mins followed by substrate addition measured up to 2 mins by fluorescence assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Aquifex aeolicus |
| Confidence | 8 — Homologous single protein target |
| Curated By | Intermediate |
Target
Phospho-N-acetylmuramoyl-pentapeptide-transferase (CHEMBL4295560) ↗| Type | SINGLE PROTEIN |
| Organism | Aquifex aeolicus (strain VF5) |
Publication
Antibacterial Muraymycins from Mutant Strains of Streptomyces sp. NRRL 30471.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 4 | 9.48 | 9.49 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4279378 | — | — | 1 | 9.49 |
| CHEMBL4286766 | — | — | 1 | 9.46 |
| CHEMBL4284923 | — | — | 1 | - |
| CHEMBL4283345 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4279378 | — | IC50 | = | 0.32 | nM | 9.49 |
| CHEMBL4286766 | — | IC50 | = | 0.35 | nM | 9.46 |
| CHEMBL4284923 | — | IC50 | = | 1.8 | 10'3pM | - |
| CHEMBL4283345 | — | IC50 | = | 0.0068 | nM | - |