Assay Detail
Binding
CHEMBL4256562
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant mouse N-terminal FLAG-tagged Notch1 (1704 to 2531 residues) expressed in human LS174T cells assessed as reduction in doxycycline-induced NICD expression after 12 hrs by Bright-Glo luciferase reporter gene assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Mus musculus |
| Cell Type | LS174T |
| Confidence | 9 — Direct single protein target |
| Curated By | Intermediate |
Target
Neurogenic locus notch homolog protein 1 (CHEMBL4295799) ↗| Type | SINGLE PROTEIN |
| Organism | Mus musculus |
Publication
The Notch Inhibitors Isolated from Nerium indicum.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 8 | 5.81 | 6.92 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4285883 | OLEANDRIN | 1.0 | 1 | 6.92 |
| CHEMBL4292704 | — | — | 1 | 5.60 |
| CHEMBL229258 | — | — | 1 | 5.57 |
| CHEMBL4282921 | — | — | 1 | 5.57 |
| CHEMBL229259 | — | — | 1 | 5.38 |
| CHEMBL4284834 | — | — | 1 | - |
| CHEMBL1453 | DIGITOXIGENIN | — | 1 | - |
| CHEMBL229260 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4285883 | OLEANDRIN | IC50 | = | 120.0 | nM | 6.92 |
| CHEMBL4292704 | — | IC50 | = | 2500.0 | nM | 5.60 |
| CHEMBL229258 | — | IC50 | = | 2700.0 | nM | 5.57 |
| CHEMBL4282921 | — | IC50 | = | 2700.0 | nM | 5.57 |
| CHEMBL229259 | — | IC50 | = | 4200.0 | nM | 5.38 |
| CHEMBL4284834 | — | IC50 | - | — | - | |
| CHEMBL1453 | DIGITOXIGENIN | IC50 | - | — | - | |
| CHEMBL229260 | — | IC50 | - | — | - |