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Assay Detail

CHEMBL4256643

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human full length N-terminal GST-tagged Aurora B (1 to 344 end residues)/His-tagged INCENP (803 to 918 end residues) expressed in baculovirus expression system using fluorescence-labeled FL-Peptide 21 as substrate after 60 mins in presence of ATP by fluorescence assay based Cheng-Prusoff equation analysis
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 7 — Homologous single protein target
Curated By Intermediate

Target

Aurora kinase B/Inner centromere protein (CHEMBL3430907)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Synthesis and profiling of a 3-aminopyridin-2-one-based kinase targeted fragment library: Identification of 3-amino-5-(pyridin-4-yl)pyridin-2(1H)-one scaffold for monopolar spindle 1 (MPS1) and Aurora kinases inhibition.
Fearon D, Westwood IM, van Montfort RLM, Bayliss R, Jones K, Bavetsias V.
Bioorg Med Chem (2018) 26 : 3021 -3029
PubMed 29764757 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 24 4.61 5.50

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4288628 1 5.50
CHEMBL4291472 1 5.44
CHEMBL4286130 1 5.26
CHEMBL1765097 1 5.13
CHEMBL4282263 1 4.92
CHEMBL4280041 1 4.89
CHEMBL4292538 1 4.82
CHEMBL4295011 1 4.73
CHEMBL302219 1 4.59
CHEMBL4292942 1 4.48
CHEMBL1738828 1 4.39
CHEMBL4280308 1 4.38
CHEMBL12856 INAMRINONE 4.0 1 4.23
CHEMBL4291350 1 4.19
CHEMBL1605269 1 4.15
CHEMBL1201292 1 4.01
CHEMBL4282410 1 3.99
CHEMBL4286709 1 3.80
CHEMBL4281678 1 -
CHEMBL4279867 1 -
CHEMBL4278218 1 -
CHEMBL4285109 1 -
CHEMBL4278538 1 -
CHEMBL189 MILRINONE 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4288628 Ki = 3200.0 nM 5.50
CHEMBL4291472 Ki = 3600.0 nM 5.44
CHEMBL4286130 Ki = 5500.0 nM 5.26
CHEMBL1765097 Ki = 7400.0 nM 5.13
CHEMBL4282263 Ki = 12000.0 nM 4.92
CHEMBL4280041 Ki = 12900.0 nM 4.89
CHEMBL4292538 Ki = 15100.0 nM 4.82
CHEMBL4295011 Ki = 18700.0 nM 4.73
CHEMBL302219 Ki = 25700.0 nM 4.59
CHEMBL4292942 Ki = 32900.0 nM 4.48
CHEMBL1738828 Ki = 41000.0 nM 4.39
CHEMBL4280308 Ki = 41700.0 nM 4.38
CHEMBL12856 INAMRINONE Ki = 59300.0 nM 4.23
CHEMBL4291350 Ki = 63900.0 nM 4.19
CHEMBL1605269 Ki = 70300.0 nM 4.15
CHEMBL1201292 Ki = 97200.0 nM 4.01
CHEMBL4282410 Ki = 102700.0 nM 3.99
CHEMBL4286709 Ki = 159700.0 nM 3.80
CHEMBL4281678 Ki > 100000.0 nM -
CHEMBL4279867 Ki > 100000.0 nM -
CHEMBL4278218 Ki > 100000.0 nM -
CHEMBL4285109 Ki > 100000.0 nM -
CHEMBL4278538 Ki > 100000.0 nM -
CHEMBL189 MILRINONE Ki > 100000.0 nM -