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Assay Detail

CHEMBL4256800

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Binding
Antagonist activity at rat TRPM8 expressed in HEK293 cells assessed as reduction in menthol-induced channel currents by whole cell patch clamp method
5
Total Activities
3
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Identification of a Potent Tryptophan-Based TRPM8 Antagonist With in Vivo Analgesic Activity.
Bertamino A, Iraci N, Ostacolo C, Ambrosino P, Musella S, Di Sarno V, Ciaglia T, Pepe G, Sala M, Soldovieri MV, Mosca I, Gonzalez-Rodriguez S, Fernandez-Carvajal A, Ferrer-Montiel A, Novellino E, Taglialatela M, Campiglia P, Gomez-Monterrey I.
J Med Chem (2018) 61 : 6140 -6152
PubMed 29939028 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 8.07 9.70
Inhibition 2 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4295040 2 9.70
CHEMBL4284808 2 8.46
CHEMBL4282067 1 6.06

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4295040 IC50 = 0.2 nM 9.70
CHEMBL4284808 IC50 = 3.5 nM 8.46
CHEMBL4282067 IC50 = 865.0 nM 6.06
CHEMBL4295040 Inhibition - % -
CHEMBL4284808 Inhibition - % -