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Assay Detail

CHEMBL4257974

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Inhibition of human recombinant CYP1B1 expressed in HEK293 cells using 7-ethoxyresorufin substrate substrate preincubated for 30 mins followed by substrate addition and measured after 60 mins by fluorescence assay relative to control
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cytochrome P450 1B1 (CHEMBL4878)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of karanjin isolated from the Indian beech tree as a potent CYP1 enzyme inhibitor with cellular efficacy <i>via</i> screening of a natural product repository.
Joshi P, Sonawane VR, Williams IS, McCann GJP, Gatchie L, Sharma R, Satti N, Chaudhuri B, Bharate SB.
Medchemcomm (2018) 9 : 371 -382
PubMed 30108931 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 5.56 6.67

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL44541 MERIDIANIN C 1 6.67
CHEMBL4282750 1 6.66
CHEMBL452751 PHELLOPTERIN 1 6.66
CHEMBL226508 1 5.60
CHEMBL4285296 1 5.22
CHEMBL295316 PLUMBAGIN 1.0 1 5.21
CHEMBL140796 ISOPIMPINELLIN 1 5.20
CHEMBL4294195 1 5.11
CHEMBL24171 BERGAPTEN 3.0 1 4.82
CHEMBL44746 KHELLIN 2.0 1 4.46
CHEMBL274056 NAPHTHAZALIN 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL44541 MERIDIANIN C IC50 = 213.0 nM 6.67
CHEMBL4282750 IC50 = 220.0 nM 6.66
CHEMBL452751 PHELLOPTERIN IC50 = 220.0 nM 6.66
CHEMBL226508 IC50 = 2500.0 nM 5.60
CHEMBL4285296 IC50 = 6040.0 nM 5.22
CHEMBL295316 PLUMBAGIN IC50 = 6150.0 nM 5.21
CHEMBL140796 ISOPIMPINELLIN IC50 = 6380.0 nM 5.20
CHEMBL4294195 IC50 = 7700.0 nM 5.11
CHEMBL24171 BERGAPTEN IC50 = 15300.0 nM 4.82
CHEMBL44746 KHELLIN IC50 = 34600.0 nM 4.46
CHEMBL274056 NAPHTHAZALIN IC50 > 10000.0 nM -