Assay Detail
Binding
CHEMBL4259631
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of TNFalpha (unknown origin)-induced adhesion of BCECF fluorescence-labeled human U937 cells to human HT-29 cells pretreated for 1 hr followed by BCECF fluorescence-labeled human U937 cell/TNFalpha addition and measured after 3 hrs by fluorescence assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Synthesis and evaluation of 6-heteroarylamino-2,4,5-trimethylpyridin-3-ols as inhibitors of TNF-α-induced cell adhesion and inflammatory bowel disease.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 4 | 5.99 | 6.54 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4284433 | — | — | 1 | 6.54 |
| CHEMBL4277438 | — | — | 1 | 6.44 |
| CHEMBL4280147 | — | — | 1 | 6.27 |
| CHEMBL704 | MESALAMINE | 4.0 | 1 | 4.70 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4284433 | — | IC50 | = | 290.0 | nM | 6.54 |
| CHEMBL4277438 | — | IC50 | = | 360.0 | nM | 6.44 |
| CHEMBL4280147 | — | IC50 | = | 540.0 | nM | 6.27 |
| CHEMBL704 | MESALAMINE | IC50 | = | 20000.0 | nM | 4.70 |