Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL4262185

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Escherichia coli DNA gyrase assessed as inhibition of pUC19 supercoiling after 30 mins by agarose gel electrophoresis
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Escherichia coli
Confidence 7 — Homologous single protein target
Curated By Intermediate

Target

DNA gyrase (CHEMBL2094139)
Type PROTEIN COMPLEX
Organism Escherichia coli

Publication

Targeting quinolone- and aminocoumarin-resistant bacteria with new gyramide analogs that inhibit DNA gyrase.
Hurley KA, Santos TMA, Fensterwald MR, Rajendran M, Moore JT, Balmond EI, Blahnik BJ, Faulkner KC, Foss MH, Heinrich VA, Lammers MG, Moore LC, Reynolds GD, Shearn-Nance GP, Stearns BA, Yao ZW, Shaw JT, Weibel DB.
Medchemcomm (2017) 8 : 942 -951
PubMed 30034678 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 7.00 7.33

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4289833 1 7.33
CHEMBL4293211 1 7.28
CHEMBL36506 NOVOBIOCIN 4.0 1 6.84
CHEMBL8 CIPROFLOXACIN 4.0 1 6.77
CHEMBL4291620 1 6.77

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4289833 IC50 = 47.0 nM 7.33
CHEMBL4293211 IC50 = 52.0 nM 7.28
CHEMBL36506 NOVOBIOCIN IC50 = 144.0 nM 6.84
CHEMBL8 CIPROFLOXACIN IC50 = 170.0 nM 6.77
CHEMBL4291620 IC50 = 170.0 nM 6.77