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Assay Detail

CHEMBL4265151

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human Ptp1B using p-nitrophenyl phosphate as substrate preincubated for 10 mins followed by substrate addition and measured every minute for 10 mins by UV-VIS spectrophotometric analysis
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein phosphatase non-receptor type 1 (CHEMBL335)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthetic thiosemicarbazones as a new class of Mycobacterium tuberculosis protein tyrosine phosphatase A inhibitors.
Sens L, de Souza ACA, Pacheco LA, Menegatti ACO, Mori M, Mascarello A, Nunes RJ, Terenzi H.
Bioorg Med Chem (2018) 26 : 5742 -5750
PubMed 30389409 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 4.74 5.03

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4288744 1 5.03
CHEMBL4292242 1 4.77
CHEMBL4282817 1 4.42
CHEMBL4280516 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4288744 IC50 = 9400.0 nM 5.03
CHEMBL4292242 IC50 = 17000.0 nM 4.77
CHEMBL4282817 IC50 = 38200.0 nM 4.42
CHEMBL4280516 IC50 > 50000.0 nM -