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Assay Detail

CHEMBL4265758

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human AKR1C1 expressed in Escherichia coli BL21 cells in presence of 9,10-phenanthrenequinone and NADPH by fluorescence assay
26
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Aldo-keto reductase family 1 member C1 (CHEMBL5905)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Screening, synthesis, crystal structure, and molecular basis of 6-amino-4-phenyl-1,4-dihydropyrano[2,3-c]pyrazole-5-carbonitriles as novel AKR1C3 inhibitors.
Zheng X, Jiang Z, Li X, Zhang C, Li Z, Wu Y, Wang X, Zhang C, Luo HB, Xu J, Wu D.
Bioorg Med Chem (2018) 26 : 5934 -5943
PubMed 30429100 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 26 5.99 6.39

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4293881 1 6.39
CHEMBL4291554 1 6.36
CHEMBL4287291 1 6.35
CHEMBL4294478 1 6.33
CHEMBL4294539 1 6.31
CHEMBL4290064 1 6.28
CHEMBL4282842 1 6.27
CHEMBL4287863 1 6.18
CHEMBL509 MECLOFENAMIC ACID 4.0 1 6.13
CHEMBL4295234 1 6.10
CHEMBL4286627 1 6.10
CHEMBL4279383 1 6.09
CHEMBL4280914 1 6.07
CHEMBL4286233 1 6.06
CHEMBL4290009 1 5.98
CHEMBL4279326 1 5.96
CHEMBL4293449 1 5.92
CHEMBL4278318 1 5.92
CHEMBL4277845 1 5.90
CHEMBL4288500 1 5.60
CHEMBL1510262 1 5.60
CHEMBL4286167 1 5.46
CHEMBL4278250 1 5.29
CHEMBL4282145 1 5.01
CHEMBL4286587 1 -
CHEMBL4293389 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4293881 IC50 = 411.0 nM 6.39
CHEMBL4291554 IC50 = 433.0 nM 6.36
CHEMBL4287291 IC50 = 443.0 nM 6.35
CHEMBL4294478 IC50 = 462.0 nM 6.33
CHEMBL4294539 IC50 = 487.0 nM 6.31
CHEMBL4290064 IC50 = 531.0 nM 6.28
CHEMBL4282842 IC50 = 534.0 nM 6.27
CHEMBL4287863 IC50 = 661.0 nM 6.18
CHEMBL509 MECLOFENAMIC ACID IC50 = 740.0 nM 6.13
CHEMBL4295234 IC50 = 801.0 nM 6.10
CHEMBL4286627 IC50 = 790.0 nM 6.10
CHEMBL4279383 IC50 = 810.0 nM 6.09
CHEMBL4280914 IC50 = 844.0 nM 6.07
CHEMBL4286233 IC50 = 870.0 nM 6.06
CHEMBL4290009 IC50 = 1050.0 nM 5.98
CHEMBL4279326 IC50 = 1090.0 nM 5.96
CHEMBL4293449 IC50 = 1200.0 nM 5.92
CHEMBL4278318 IC50 = 1210.0 nM 5.92
CHEMBL4277845 IC50 = 1270.0 nM 5.90
CHEMBL4288500 IC50 = 2520.0 nM 5.60
CHEMBL1510262 IC50 = 2490.0 nM 5.60
CHEMBL4286167 IC50 = 3510.0 nM 5.46
CHEMBL4278250 IC50 = 5090.0 nM 5.29
CHEMBL4282145 IC50 = 9870.0 nM 5.01
CHEMBL4286587 IC50 > 25000.0 nM -
CHEMBL4293389 IC50 > 25000.0 nM -