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Assay Detail

CHEMBL4266473

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of IRAK4 in human KARPAS299 cells assessed as reduction in IL-1 stimulated IRAK4 phosphorylation at Thr345/Ser346 residues preincubated for 1 hr followed by IL-1 stimulation for 10 mins by flow cytometry analysis
27
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Interleukin-1 receptor-associated kinase 4 (CHEMBL3778)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Optimization of permeability in a series of pyrrolotriazine inhibitors of IRAK4.
Degorce SL, Anjum R, Dillman KS, Drew L, Groombridge SD, Halsall CT, Lenz EM, Lindsay NA, Mayo MF, Pink JH, Robb GR, Scott JS, Stokes S, Xue Y.
Bioorg Med Chem (2018) 26 : 913 -924
PubMed 29398441 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 27 6.13 6.86

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4280559 2 6.86
CHEMBL4293607 2 6.85
CHEMBL4278882 2 6.71
CHEMBL4294023 2 6.57
CHEMBL4285338 2 6.48
CHEMBL4288305 2 6.45
CHEMBL4286802 2 6.44
CHEMBL4290222 2 6.38
CHEMBL4279192 1 6.12
CHEMBL4282348 2 5.78
CHEMBL4290202 2 5.70
CHEMBL4283453 2 5.63
CHEMBL4280028 2 5.05
CHEMBL4292700 2 4.87

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4280559 IC50 = 138.04 nM 6.86
CHEMBL4293607 IC50 = 141.0 nM 6.85
CHEMBL4293607 IC50 = 141.25 nM 6.85
CHEMBL4280559 IC50 = 140.0 nM 6.85
CHEMBL4278882 IC50 = 195.0 nM 6.71
CHEMBL4278882 IC50 = 194.98 nM 6.71
CHEMBL4294023 IC50 = 267.0 nM 6.57
CHEMBL4294023 IC50 = 269.15 nM 6.57
CHEMBL4285338 IC50 = 331.0 nM 6.48
CHEMBL4285338 IC50 = 331.13 nM 6.48
CHEMBL4288305 IC50 = 359.0 nM 6.45
CHEMBL4286802 IC50 = 363.08 nM 6.44
CHEMBL4286802 IC50 = 361.0 nM 6.44
CHEMBL4288305 IC50 = 363.08 nM 6.44
CHEMBL4290222 IC50 = 420.0 nM 6.38
CHEMBL4290222 IC50 = 416.87 nM 6.38
CHEMBL4279192 IC50 = 758.0 nM 6.12
CHEMBL4282348 IC50 = 1659.59 nM 5.78
CHEMBL4282348 IC50 = 1650.0 nM 5.78
CHEMBL4290202 IC50 = 1990.0 nM 5.70
CHEMBL4290202 IC50 = 1995.26 nM 5.70
CHEMBL4283453 IC50 = 2344.23 nM 5.63
CHEMBL4283453 IC50 = 2340.0 nM 5.63
CHEMBL4280028 IC50 = 8910.0 nM 5.05
CHEMBL4280028 IC50 = 8912.51 nM 5.05
CHEMBL4292700 IC50 = 13489.63 nM 4.87
CHEMBL4292700 IC50 = 13600.0 nM 4.87