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Assay Detail

CHEMBL4266977

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant N-terminal His6-tagged human Pin1 using Suc-Ala-Glu-cis-Pro-Phe-4-nitroanilide as substrate preincubated for 30 mins followed by substrate addition measured for 90 secs
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (CHEMBL2288)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis and biological evaluation of ring A modified 11-keto-boswellic acid derivatives as Pin1 inhibitors with remarkable anti-prostate cancer activity.
Huang M, Li A, Zhao F, Xie X, Li K, Jing Y, Liu D, Zhao L.
Bioorg Med Chem Lett (2018) 28 : 3187 -3193
PubMed 30153964 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 5.73 6.34

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4283818 1 6.34
CHEMBL4278419 1 6.26
CHEMBL4294741 1 6.17
CHEMBL4287574 1 6.01
CHEMBL4287705 1 6.00
CHEMBL4292071 1 5.90
CHEMBL4276931 1 5.80
CHEMBL4280911 1 5.66
CHEMBL4289090 1 5.57
CHEMBL43612 JUGLONE 1 5.00
CHEMBL237111 AKBA -1.0 1 4.30

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4283818 IC50 = 460.0 nM 6.34
CHEMBL4278419 IC50 = 550.0 nM 6.26
CHEMBL4294741 IC50 = 680.0 nM 6.17
CHEMBL4287574 IC50 = 970.0 nM 6.01
CHEMBL4287705 IC50 = 1010.0 nM 6.00
CHEMBL4292071 IC50 = 1270.0 nM 5.90
CHEMBL4276931 IC50 = 1580.0 nM 5.80
CHEMBL4280911 IC50 = 2180.0 nM 5.66
CHEMBL4289090 IC50 = 2680.0 nM 5.57
CHEMBL43612 JUGLONE IC50 = 10030.0 nM 5.00
CHEMBL237111 AKBA IC50 = 50360.0 nM 4.30