Assay Detail
Binding
CHEMBL4267725
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant human C-terminal His6-tagged FPPS expressed in Escherichia coli BL21 using DMAPP and IPP as substrates pretreated for 15 mins followed by substrates addition and measured after 1 hr by colorimetric method
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Novel bisphosphonates with antiresorptive effect in bone mineralization and osteoclastogenesis.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 4 | 6.32 | 7.22 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4303669 | ZOLEDRONIC ACID | 4.0 | 1 | 7.22 |
| CHEMBL923 | RISEDRONIC ACID | 4.0 | 1 | 7.16 |
| CHEMBL4283512 | — | — | 1 | 6.51 |
| CHEMBL4279995 | — | — | 1 | 4.39 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4303669 | ZOLEDRONIC ACID | IC50 | = | 60.0 | nM | 7.22 |
| CHEMBL923 | RISEDRONIC ACID | IC50 | = | 70.0 | nM | 7.16 |
| CHEMBL4283512 | — | IC50 | = | 310.0 | nM | 6.51 |
| CHEMBL4279995 | — | IC50 | = | 41000.0 | nM | 4.39 |