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Assay Detail

CHEMBL4268906

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human kidneyl SGLT2 expressed in CHOK1 cells assessed as reduction in Na-dependent [14C]-AMG uptake preincubated for 10 mins followed by [14C]-AMG addition measured after 2 hrs by TopCount method
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium/glucose cotransporter 2 (CHEMBL3884)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis and biological evaluation of (2S,3R,4R,5S,6R)-5-fluoro-6-(hydroxymethyl)-2-aryltetrahydro-2H-pyran-3,4-diols as potent and orally active SGLT dual inhibitors.
Xu G, Gaul MD, Kuo GH, Du F, Xu JZ, Wallace N, Hinke S, Kirchner T, Silva J, Huebert ND, Lee S, Murray W, Liang Y, Demarest K.
Bioorg Med Chem Lett (2018) 28 : 3446 -3453
PubMed 30268701 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 7.99 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4281478 1 8.70
CHEMBL4294285 1 8.59
CHEMBL4291795 1 8.52
CHEMBL4286399 1 8.28
CHEMBL4293989 1 8.12
CHEMBL4289884 1 8.08
CHEMBL4279519 1 8.05
CHEMBL4279564 1 7.87
CHEMBL4285963 1 7.63
CHEMBL4285800 1 7.44
CHEMBL4287888 1 7.44
CHEMBL4282708 1 7.21

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4281478 IC50 = 2.0 nM 8.70
CHEMBL4294285 IC50 = 2.6 nM 8.59
CHEMBL4291795 IC50 = 3.0 nM 8.52
CHEMBL4286399 IC50 = 5.3 nM 8.28
CHEMBL4293989 IC50 = 7.5 nM 8.12
CHEMBL4289884 IC50 = 8.3 nM 8.08
CHEMBL4279519 IC50 = 9.0 nM 8.05
CHEMBL4279564 IC50 = 13.6 nM 7.87
CHEMBL4285963 IC50 = 23.2 nM 7.63
CHEMBL4285800 IC50 = 36.0 nM 7.44
CHEMBL4287888 IC50 = 36.0 nM 7.44
CHEMBL4282708 IC50 = 62.3 nM 7.21