Assay Detail
Binding
CHEMBL4273764
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of mouse full length GST-tagged BRAF V600E mutant using recombinant human His6-tagged MEK1 as substrate preincubated for 1 hr followed by substrate addition measured after 25 mins by ELISA
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Mus musculus |
| Confidence | 9 — Direct single protein target |
| Curated By | Intermediate |
Target
Serine/threonine-protein kinase B-raf (CHEMBL2331061) ↗| Type | SINGLE PROTEIN |
| Organism | Mus musculus |
Publication
Synthesis, anticancer activity and molecular modeling studies of 1,2,4-triazole derivatives as EGFR inhibitors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 2 | 6.37 | 7.40 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1079742 | ERLOTINIB HYDROCHLORIDE | 4.0 | 1 | 7.40 |
| CHEMBL4279008 | — | — | 1 | 5.33 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1079742 | ERLOTINIB HYDROCHLORIDE | IC50 | = | 40.0 | nM | 7.40 |
| CHEMBL4279008 | — | IC50 | = | 4700.0 | nM | 5.33 |