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Assay Detail

CHEMBL4274201

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human EGFR L858R mutant expressed in baculovirus infected insect cells preincubated for 5 mins followed by ATP addition and measured after 30 mins by HTRF assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis, antiproliferative activity, molecular docking and cell cycle analysis of some novel (morpholinosulfonyl) isatins with potential EGFR inhibitory activity.
Ammar YA, Sh El-Sharief AM, Belal A, Abbas SY, Mohamed YA, Mehany ABM, Ragab A.
Eur J Med Chem (2018) 156 : 918 -932
PubMed 30096580 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 7.61 7.94

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL939 GEFITINIB 4.0 1 7.94
CHEMBL4282506 1 7.47
CHEMBL554 LAPATINIB 4.0 1 7.42
CHEMBL553 ERLOTINIB 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL939 GEFITINIB IC50 = 11.5 nM 7.94
CHEMBL4282506 IC50 = 33.8 nM 7.47
CHEMBL554 LAPATINIB IC50 = 37.55 nM 7.42
CHEMBL553 ERLOTINIB IC50 - -