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Assay Detail

CHEMBL4305684

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human SIRT2 defatty-acylase activity using SFP3 as substrate measured at 5 mins interval for 60 mins in presence of NAD+ by fluorescence assay
21
Total Activities
21
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

NAD-dependent protein deacetylase sirtuin-2 (CHEMBL4462)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development of Peptide-Based Sirtuin Defatty-Acylase Inhibitors Identified by the Fluorescence Probe, SFP3, That Can Efficiently Measure Defatty-Acylase Activity of Sirtuin.
Kawaguchi M, Ieda N, Nakagawa H.
J Med Chem (2019) 62 : 5434 -5452
PubMed 31117516 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 6.83 7.72
Inhibition 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4447813 1 7.72
CHEMBL4446458 1 7.72
CHEMBL4438193 1 7.66
CHEMBL4457496 1 7.44
CHEMBL4529328 1 7.20
CHEMBL4461872 1 7.17
CHEMBL4553811 1 6.92
CHEMBL4449389 1 6.52
CHEMBL4458848 1 6.50
CHEMBL4448993 1 6.37
CHEMBL4445565 1 5.82
CHEMBL1140 NIACINAMIDE 4.0 1 4.96
CHEMBL4559310 1 -
CHEMBL3770903 1 -
CHEMBL4590449 1 -
CHEMBL4129995 1 -
CHEMBL4439944 1 -
CHEMBL4464941 1 -
CHEMBL4465910 1 -
CHEMBL4457681 1 -
CHEMBL4574491 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4447813 IC50 = 19.0 nM 7.72
CHEMBL4446458 IC50 = 19.0 nM 7.72
CHEMBL4438193 IC50 = 22.0 nM 7.66
CHEMBL4457496 IC50 = 36.0 nM 7.44
CHEMBL4529328 IC50 = 63.0 nM 7.20
CHEMBL4461872 IC50 = 68.0 nM 7.17
CHEMBL4553811 IC50 = 120.0 nM 6.92
CHEMBL4449389 IC50 = 300.0 nM 6.52
CHEMBL4458848 IC50 = 320.0 nM 6.50
CHEMBL4448993 IC50 = 430.0 nM 6.37
CHEMBL4445565 IC50 = 1500.0 nM 5.82
CHEMBL1140 NIACINAMIDE IC50 = 11000.0 nM 4.96
CHEMBL4559310 Inhibition - % -
CHEMBL3770903 IC50 > 10000.0 nM -
CHEMBL4590449 IC50 > 50000.0 nM -
CHEMBL4129995 IC50 > 2000.0 nM -
CHEMBL4439944 IC50 > 2000.0 nM -
CHEMBL4464941 IC50 > 2000.0 nM -
CHEMBL4465910 IC50 > 2000.0 nM -
CHEMBL4457681 IC50 > 2000.0 nM -
CHEMBL4574491 IC50 > 2000.0 nM -