Assay Detail
Binding
CHEMBL4305709
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant human SIRT3 (118 to 399 residues) demyristoylase activity expressed in Escherichia coli using p53(Myr)-AMC as substrate measured after 3 hrs in presence of NAD+ and trypsin by fluorescence assay
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
NAD-dependent protein deacetylase sirtuin-3, mitochondrial (CHEMBL4461) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Development of Peptide-Based Sirtuin Defatty-Acylase Inhibitors Identified by the Fluorescence Probe, SFP3, That Can Efficiently Measure Defatty-Acylase Activity of Sirtuin.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 9 | 6.58 | 6.89 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4438193 | — | — | 1 | 6.89 |
| CHEMBL4446458 | — | — | 1 | 6.72 |
| CHEMBL4457496 | — | — | 1 | 6.48 |
| CHEMBL4447813 | — | — | 1 | 6.47 |
| CHEMBL4461872 | — | — | 1 | 6.32 |
| CHEMBL4529328 | — | — | 1 | - |
| CHEMBL4553811 | — | — | 1 | - |
| CHEMBL1140 | NIACINAMIDE | 4.0 | 1 | - |
| CHEMBL3770903 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4438193 | — | IC50 | = | 130.0 | nM | 6.89 |
| CHEMBL4446458 | — | IC50 | = | 190.0 | nM | 6.72 |
| CHEMBL4457496 | — | IC50 | = | 330.0 | nM | 6.48 |
| CHEMBL4447813 | — | IC50 | = | 340.0 | nM | 6.47 |
| CHEMBL4461872 | — | IC50 | = | 480.0 | nM | 6.32 |
| CHEMBL4529328 | — | IC50 | - | — | - | |
| CHEMBL4553811 | — | IC50 | - | — | - | |
| CHEMBL1140 | NIACINAMIDE | IC50 | - | — | - | |
| CHEMBL3770903 | — | IC50 | - | — | - |