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Assay Detail

CHEMBL4305722

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human SIRT2 deacylation activity expressed in Escherichia coli Rosetta2 using Fluor-de-Lys-SIRT2 deacetylase substrate measured after 2 hrs by fluorescence assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

NAD-dependent protein deacetylase sirtuin-2 (CHEMBL4462)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development of Peptide-Based Sirtuin Defatty-Acylase Inhibitors Identified by the Fluorescence Probe, SFP3, That Can Efficiently Measure Defatty-Acylase Activity of Sirtuin.
Kawaguchi M, Ieda N, Nakagawa H.
J Med Chem (2019) 62 : 5434 -5452
PubMed 31117516 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 7.51 7.51

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4458848 1 7.51

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4458848 IC50 = 31.0 nM 7.51