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Assay Detail

CHEMBL4307198

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]-di-o-tolylguanidine from sigma2 receptor in rat liver membranes in the presence of sigma1 receptor ligand (+)-pentazocine incubated for 120 mins by scintillation counting method
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Liver
Subcellular Membranes
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sigma intracellular receptor 2 (CHEMBL4105864)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Discovery of new potent dual sigma receptor/GluN2b ligands with antioxidant property as neuroprotective agents.
Zampieri D, Fortuna S, Calabretti A, Romano M, Menegazzi R, Schepmann D, Wünsch B, Collina S, Zanon D, Mamolo MG.
Eur J Med Chem (2019) 180 : 268 -282
PubMed 31319263 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 17 7.12 8.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4581662 1 8.82
CHEMBL4446466 1 7.80
CHEMBL4449269 1 7.68
CHEMBL4446431 1 7.68
CHEMBL4533030 1 7.50
CHEMBL4459690 1 7.47
CHEMBL4439170 1 7.35
CHEMBL282433 DITOLYLGUANIDINE 1 7.24
CHEMBL4453315 1 7.14
CHEMBL54 HALOPERIDOL 4.0 1 7.11
CHEMBL4463741 1 7.10
CHEMBL4451677 1 7.03
CHEMBL4445874 1 7.01
CHEMBL4454333 1 6.42
CHEMBL4567801 1 6.16
CHEMBL4446009 1 6.06
CHEMBL4442750 1 5.55

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4581662 Ki = 1.5 nM 8.82
CHEMBL4446466 Ki = 16.0 nM 7.80
CHEMBL4449269 Ki = 21.0 nM 7.68
CHEMBL4446431 Ki = 21.0 nM 7.68
CHEMBL4533030 Ki = 32.0 nM 7.50
CHEMBL4459690 Ki = 34.0 nM 7.47
CHEMBL4439170 Ki = 45.0 nM 7.35
CHEMBL282433 DITOLYLGUANIDINE Ki = 57.0 nM 7.24
CHEMBL4453315 Ki = 73.0 nM 7.14
CHEMBL54 HALOPERIDOL Ki = 78.0 nM 7.11
CHEMBL4463741 Ki = 80.0 nM 7.10
CHEMBL4451677 Ki = 93.0 nM 7.03
CHEMBL4445874 Ki = 98.0 nM 7.01
CHEMBL4454333 Ki = 383.0 nM 6.42
CHEMBL4567801 Ki = 686.0 nM 6.16
CHEMBL4446009 Ki = 864.0 nM 6.06
CHEMBL4442750 Ki = 2800.0 nM 5.55