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Assay Detail

CHEMBL4308356

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Cathepsin L (unknown origin) using Z-Phe-Arg-7-amido-4-methylcoumarin as substrate preincubated for 2 mins followed by susbtrate addition by fluorescence based analysis
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Procathepsin L (CHEMBL3837)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and structure-activity relationship of nitrile-based cruzain inhibitors incorporating a trifluoroethylamine-based P2 amide replacement.
Gomes JC, Cianni L, Ribeiro J, Dos Reis Rocho F, da Costa Martins Silva S, Batista PHJ, Moraes CB, Franco CH, Freitas-Junior LHG, Kenny PW, Leitão A, Burtoloso ACB, de Vita D, Montanari CA.
Bioorg Med Chem (2019) 27 : 115083 -115083
PubMed 31561938 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 19 6.57 8.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4451757 1 8.30
CHEMBL4442543 1 7.70
CHEMBL4473385 1 7.60
CHEMBL4463665 1 7.40
CHEMBL4578891 1 7.20
CHEMBL4544254 1 6.80
CHEMBL4455305 1 6.60
CHEMBL4446278 1 6.60
CHEMBL4440148 1 6.20
CHEMBL4546133 1 6.20
CHEMBL4586752 1 6.20
CHEMBL4579894 1 6.10
CHEMBL4515535 1 6.00
CHEMBL4543467 1 6.00
CHEMBL481611 ODANACATIB 3.0 1 5.90
CHEMBL4535973 1 5.90
CHEMBL4565840 1 5.80
CHEMBL4456233 1 5.80
CHEMBL4441186 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4451757 Ki = 5.012 nM 8.30
CHEMBL4442543 Ki = 19.95 nM 7.70
CHEMBL4473385 Ki = 25.12 nM 7.60
CHEMBL4463665 Ki = 39.81 nM 7.40
CHEMBL4578891 Ki = 63.1 nM 7.20
CHEMBL4544254 Ki = 158.49 nM 6.80
CHEMBL4455305 Ki = 251.19 nM 6.60
CHEMBL4446278 Ki = 251.19 nM 6.60
CHEMBL4440148 Ki = 630.96 nM 6.20
CHEMBL4546133 Ki = 630.96 nM 6.20
CHEMBL4586752 Ki = 630.96 nM 6.20
CHEMBL4579894 Ki = 794.33 nM 6.10
CHEMBL4515535 Ki = 1000.0 nM 6.00
CHEMBL4543467 Ki = 1000.0 nM 6.00
CHEMBL481611 ODANACATIB Ki = 1258.93 nM 5.90
CHEMBL4535973 Ki = 1258.93 nM 5.90
CHEMBL4565840 Ki = 1584.89 nM 5.80
CHEMBL4456233 Ki = 1584.89 nM 5.80
CHEMBL4441186 Ki > 10000.0 nM -