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Assay Detail

CHEMBL4308796

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of fluorescent labelled fluormone ES2 from human recombinant GST tagged ER alpha LBD ( 282 to 595 residues) expressed in baculovirus infected insect cells measured after 20 mins by TR-FRET Lanthascreen assay
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Estrogen receptor (CHEMBL206)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Building Bridges in a Series of Estrogen Receptor Degraders: An Application of Metathesis in Medicinal Chemistry.
Scott JS, Breed J, Carbajo RJ, Davey PR, Greenwood R, Huynh HK, Klinowska T, Morrow CJ, Moss TA, Polanski R, Nissink JWM, Varnes J, Yang B.
ACS Med Chem Lett (2019) 10 : 1492 -1497
PubMed 31620239 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 9.07 9.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4447306 1 9.40
CHEMBL4587197 1 9.20
CHEMBL3623004 AZD-9496 1.0 1 9.10
CHEMBL4452972 1 9.10
CHEMBL4528514 1 9.00
CHEMBL4591926 1 9.00
CHEMBL4516412 1 8.70

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4447306 IC50 = 0.3981 nM 9.40
CHEMBL4587197 IC50 = 0.631 nM 9.20
CHEMBL3623004 AZD-9496 IC50 = 0.7943 nM 9.10
CHEMBL4452972 IC50 = 0.7943 nM 9.10
CHEMBL4528514 IC50 = 1.0 nM 9.00
CHEMBL4591926 IC50 = 1.0 nM 9.00
CHEMBL4516412 IC50 = 1.995 nM 8.70