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Assay Detail

CHEMBL4308797

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Induction of ERalpha degradation in human MCF7 cells assessed as downregulation of ER alpha receptor expression measured after 18 to 22 hrs by immunofluorescence assay
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type MCF7
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Estrogen receptor (CHEMBL206)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Building Bridges in a Series of Estrogen Receptor Degraders: An Application of Metathesis in Medicinal Chemistry.
Scott JS, Breed J, Carbajo RJ, Davey PR, Greenwood R, Huynh HK, Klinowska T, Morrow CJ, Moss TA, Polanski R, Nissink JWM, Varnes J, Yang B.
ACS Med Chem Lett (2019) 10 : 1492 -1497
PubMed 31620239 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 9.06 10.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4528514 1 10.10
CHEMBL4591926 1 9.60
CHEMBL3623004 AZD-9496 1.0 1 9.50
CHEMBL4447306 1 8.90
CHEMBL4587197 1 8.80
CHEMBL4516412 1 8.70
CHEMBL4452972 1 7.80

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4528514 IC50 = 0.07943 nM 10.10
CHEMBL4591926 IC50 = 0.2512 nM 9.60
CHEMBL3623004 AZD-9496 IC50 = 0.3162 nM 9.50
CHEMBL4447306 IC50 = 1.259 nM 8.90
CHEMBL4587197 IC50 = 1.585 nM 8.80
CHEMBL4516412 IC50 = 1.995 nM 8.70
CHEMBL4452972 IC50 = 15.85 nM 7.80