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Assay Detail

CHEMBL4311925

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at PAR2 in human EAhy926 cells assessed as inhibition of trypsin-induced intracellular calcium mobilization preincubated for 15 mins followed by trypsin addition by Fluo-4-AM dye based fluorescence assay
3
Total Activities
3
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Proteinase-activated receptor 2 (CHEMBL5963)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design and Evaluation of Heterobivalent PAR1-PAR2 Ligands as Antagonists of Calcium Mobilization.
Majewski MW, Gandhi DM, Rosas R, Kodali R, Arnold LA, Dockendorff C.
ACS Med Chem Lett (2019) 10 : 121 -126
PubMed 30655958 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Inhibition 2 - -
IC50 1 8.70 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4470628 1 8.70
CHEMBL4540591 1 -
CHEMBL4588926 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4470628 IC50 = 2.0 nM 8.70
CHEMBL4540591 Inhibition - % -
CHEMBL4588926 Inhibition - % -