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Assay Detail

CHEMBL4312904

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Competitive reversible inhibition of N-terminal His6-tagged human CDC25B (377 to 550 residues) expressed in Escherichia coli BL21(DE3) cells using OMFP as substrate by double reciprocal Lineweaver-Burk plot analysis
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

M-phase inducer phosphatase 2 (CHEMBL4804)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Novel Naphthylphenylketone and Naphthylphenylamine Derivatives as Cell Division Cycle 25B (CDC25B) Phosphatase Inhibitors: Design, Synthesis, Inhibition Mechanism, and in Vitro Efficacy against Melanoma Cell Lines.
Cerchia C, Nasso R, Mori M, Villa S, Gelain A, Capasso A, Aliotta F, Simonetti M, Rullo R, Masullo M, De Vendittis E, Ruocco MR, Lavecchia A.
J Med Chem (2019) 62 : 7089 -7110
PubMed 31294975 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 1 5.28 5.28

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1414990 1 5.28

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1414990 Ki = 5300.0 nM 5.28