Assay Detail
Binding
CHEMBL4313178
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human His6-tagged ERCC1-XPF endonuclease activity expressed in Escherichia coli BL21 (DE3) using [6-FAM-5'-CAGCGCTCGG(20T)CCGAGCGCTG-3'-dabcyl] as substrate measured after 30 mins in presence of 5% DMSO by fluorescence assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 7 — Homologous single protein target |
| Curated By | Autocuration |
Target
DNA excision repair protein ERCC-1/DNA repair endonuclease XPF (CHEMBL3883316) ↗| Type | PROTEIN COMPLEX |
| Organism | Homo sapiens |
Publication
Targeting DNA Repair in Tumor Cells via Inhibition of ERCC1-XPF.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 2 | 6.11 | 6.48 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4470176 | — | — | 1 | 6.48 |
| CHEMBL463783 | — | — | 1 | 5.73 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4470176 | — | IC50 | = | 330.0 | nM | 6.48 |
| CHEMBL463783 | — | IC50 | = | 1860.0 | nM | 5.73 |