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Assay Detail

CHEMBL4313225

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of Alexa Fluor 647 labelled ligand from His6/FLAG-tagged TEV fused BRDT BD2(1 to 397 residues)/BD2 Y66A mutant (unknown origin) after 30 mins by TR-FRET assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bromodomain testis-specific protein (CHEMBL1795185)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

A Qualified Success: Discovery of a New Series of ATAD2 Bromodomain Inhibitors with a Novel Binding Mode Using High-Throughput Screening and Hit Qualification.
Bamborough P, Chung CW, Demont EH, Bridges AM, Craggs PD, Dixon DP, Francis P, Furze RC, Grandi P, Jones EJ, Karamshi B, Locke K, Lucas SCC, Michon AM, Mitchell DJ, Pogány P, Prinjha RK, Rau C, Roa AM, Roberts AD, Sheppard RJ, Watson RJ.
J Med Chem (2019) 62 : 7506 -7525
PubMed 31398032 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 4.80 4.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4583297 1 4.80

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4583297 IC50 = 15848.93 nM 4.80